Duplex PCR method for rapid detection of Zymomonas mobilis in cider

“Framboisé” spoilage in French ciders: Zymomonas mobilis implication and characterization

Abstract Biodiversity of 17 Zymomonas mobilis strains isolated from French “framboise” ciders from 7 different geographical areas of France was analysed using random amplified polymorphic DNA (RAPD) and 6 different strain fingerprints were observed. One of the fingerprints was identical for 10 of the 17 cases. No correlation between geographical origin and strain fingerprint was observed. In parallel, the cider-making process was followed from the orchard to the final product for Z. mobilis presence. Detection of the bacterium only occurred during the fermentation process. The French isolate, strain AN0101, recently proposed as a novel subspecies ( Z. mobilis subsp. francensis ) was charact…