0000000000243406
AUTHOR
Lúcia Guilhermino
Use of atropine-treated Daphnia magna survival for detection of environmental contamination by acetylcholinesterase inhibitors.
The toxicity of cholinesterase-inhibiting compounds (e.g., carbamates and organophosphates) is due to a decrease in acetylcholine metabolism, which results in a continuous stimulation of cholinergic receptors (muscarinic and nicotinic) that can be fatal. The goal of this study was to evaluate the protective effect of atropine (muscarinic receptor antagonist) against paraoxon-induced toxicity to Daphnia magna using its survival rate for the detection of environmental contamination by cholinesterase-inhibiting compounds. As expected, paraoxon was lethal to D. magna in a concentration-dependent manner. Noteworthy, the pretreatment of these organisms with atropine dramatically increased their s…
Effect of sublethal concentrations of copper sulphate on seabreamSparus auratafingerlings
The gilthead seabream is the most important Mediterranean aquacultured fish species. The main objective of this study was to investigate whether copper sulphate bath treatments used routinely in aquaculture have effects on important physiological functions of early life stages of the gilthead seabream (Sparus aurata). Fingerlings (80-90 days, 0.27 ± 0.06 g) were exposed to copper sulphate baths at 0, 0.25, 0.5 and 1.5 mg L −1 during 24 h. Effects on the central nervous function were evaluated analysing brain acetylcholinesterase activity (AChE). Oxidative stress was assessed by the quantification of lipid peroxidation (LP). Heat shock proteins (HSP70) were used as a general response to chem…
Effects of dichlorvos aquaculture treatments on selected biomarkers of gilthead sea bream (Sparus aurata L.) fingerlings
Abstract The gilthead sea bream ( Sparus aurata ) is the most important marine cultured species in the Mediterranean. Dichlorvos is one of the main chemical agents used in bath treatments against ectoparasites of marine farmed fish. The main objective of this study was to investigate the effects of 24 h dichlorvos baths on selected biomarkers that are involved in important physiological functions or indicative of gilthead sea bream fingerlings growth. To attain this objective, the in vivo effects of dichlorvos on cholinesterases' activity (ChE), lipid peroxidation (TBARS), RNA/DNA ratio, glutathione S -transferases activity (GST) and heat shock proteins HSP70 were studied. The characterizat…