Author: Fabienne Guillon

0000000000243431

AUTHOR

Fabienne Guillon

Firming of fruit tissues by vacuum infusion of pectin methylesterase: Visualisation of enzyme action

; Apple pieces were vacuum-impregnated with either a pectin methylesterase (PME) and calcium solution or with water prior to pasteurization. Pasteurized apple pieces impregnated with PME and calcium showed a significantly higher firmness. Moreover, solid state C-13 NMR spectroscopy of apple cell wall residues revealed an increase of their molecular rigidity. Exogenous PME addition involved a decrease from 82% to 45% of apple pectin degree of methyl-esterification. Microscopic observations of apple slices immunolabelled with antibodies specific for pectins showed that (i) demethyl-esterification was more intense in the cell wall region lining intercellular spaces (demonstrating a key role f…

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Impact de la diversité génétique sur la construction des parois dans une collection de graines de légumineuses

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