0000000000243718

AUTHOR

K. Schön-hölz

showing 2 related works from this author

Occurence of vancomycin-resistant Enterococcus faecium in patient isolates and aquatic environment during a period between 2004 and 2010

2012

Microbiology (medical)Infectious DiseasesAquatic environmentPeriod (gene)educationIn patientGeneral MedicineBiologyMicrobiologyVancomycin resistant Enterococcus faeciumInternational Journal of Infectious Diseases
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Molecular monitoring of inactivation efficiencies of bacteria during pulsed electric field treatment of clinical wastewater

2008

Aims:  The applicability of an alternative wastewater disinfection concept based on the pulsed electric field (PEF) treatment is tested with molecular biology techniques using clinical wastewaters. Methods and Results:  Hospital wastewater was treated with the PEF technology. The inactivation efficiencies of bacteria were successfully monitored with real-time polymerase chain reaction (PCR). As the differentiation between living and dead bacterial cells is important for the determination of the disinfection efficiency, propidium monoazide (PMA) was applied. PMA selectively penetrates cells with compromised membranes and intercalates into the DNA inhibiting a subsequent PCR amplification. Th…

Electrophoresis Agar GelGel electrophoresisDisinfection methodsChromatographyBacteriaReverse Transcriptase Polymerase Chain ReactionColony Count MicrobialBacterial populationGeneral MedicineBiologybiology.organism_classificationPolymerase Chain ReactionWaste Disposal FluidApplied Microbiology and BiotechnologyElectric StimulationHospitalsIntercalating AgentsWater PurificationMicrobiologyDisinfectionWastewaterPropidium monoazideBacteriaPropidiumBiotechnologyJournal of Applied Microbiology
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