0000000000245240
AUTHOR
Jérôme Verdier
Use of translational genomics to identify genes important for legume seed filling
International audience; Translational genomics, i.e., the transfer of genetic information from model species to cultivated crops, is on the brink of revolutionizing plant breeding. The recent publication of genomic sequences for several cultivated legumes is also accelerating this process. For pea, recent highthroughput RNA sequencing, and the prospect of a genome sequencing project, will further accelerate the transfer of information from the Medicago truncatula model to the cultivated crop. We have been using genomics approaches with Medicago as a tool to identify key genes determining seed yield and composition in closely related legumes. Analyses of the proteome and transcriptome of the…
Regulation of legume seed size by an endosperm-expressed transcription factor
BAP Pôle GEAPSI; International audience; There are numerous reports of transcription factors (TFs) which are implicated in the control of seed size and seed composition. We have identified, using a platform of TF sequences derived from the Medicago truncatula genome sequence, a class of TFs specifically expressed during the seed filling stage. One such TF, DASH, was shown to be confined to the developing endosperm. We investigated the role played by DASH through analysis of mutant alleles. These give rise to seed-lethal or near-lethal phenotypes, with degeneration of the endosperm and arrested embryo development. The relation of this phenotype to seed auxin action was investigated.