0000000000246812
AUTHOR
Ohm D. Krishna
A Versatile Grafting-to Approach for the Bioconjugation of Polymers to Collagen-like Peptides Using an Activated Ester Chain Transfer Agent
DepartmentofMaterials Science and Engineering, University of Delaware, 201DuPont Hall, Newark, Delaware 19716,Received February 24, 2009Revised Manuscript Received April 24, 2009Biohybrid materials consisting of synthetic polymers andbiological moieties have gained more and more interest in therecent years.
Morphological transformations in a dually thermoresponsive coil-rod-coil bioconjugate.
We report the conformational and assembly behavior of a thermoresponsive triblock biohybrid conjugate under aqueous conditions. The triblock comprises of poly(diethylene glycol methyl ether methacrylate) (PDEGMEMA) conjugated to the ends of a triple-helix forming collagen-like peptide. The circular dichroism (CD) experiment confirms the ability of the collagen-like peptide middle block to assemble as a triple helix in the hybrid conjugate. Above the LCST (∼35 °C), the collapse of the thermoresponsive PDEGMEMA polymer at the ends of the peptide domain resulted in a concomitant increase in the conformational stability of the peptide domain towards thermal denaturation. Upon cooling back, the …