0000000000256471
AUTHOR
Véronique Drouineaud
Additional file 1: Table S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
Patients' characteristics. All values are reported by the mean ± SD (Standard Deviation) or the number of subjects (percentage of the total). BMI, Body Mass Index; IVF, In Vitro Fertilization; ICSI, Intracytoplasmic Sperm Injection. (DOCX 12 kb)
Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
Background Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development. Methods This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure. Apolipoprotein B (APOB) concentrations in follicular fluid and APOB gene and protein expression in granulosa cells from rep…
Additional file 3: Figure S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
17 β-estradiol quantification (ng/l) into culture medium by immuno-chemiluminescence. Androstenedione (And) was added to the culture medium to provide specific substrate to granulosa cells, for oestrogen synthesis. (TIF 69 kb)
Additional file 3: Figure S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
17 β-estradiol quantification (ng/l) into culture medium by immuno-chemiluminescence. Androstenedione (And) was added to the culture medium to provide specific substrate to granulosa cells, for oestrogen synthesis. (TIF 69 kb)
Additional file 4: Figure S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
The constant GAPDH gene expression in GC pools (n = 10) under our experiment conditions. All Ct-values were reported for the different GC pools from FF samples. (JPG 137 kb)
Additional file 4: Figure S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes
The constant GAPDH gene expression in GC pools (n = 10) under our experiment conditions. All Ct-values were reported for the different GC pools from FF samples. (JPG 137 kb)