0000000000276333

AUTHOR

P. N. Richard Dekhuijzen

showing 3 related works from this author

Oxidative and nitrosative stress in the diaphragm of patients with COPD.

2007

COPD is associated with an increased load on the diaphragm. Since chronic muscle loading results in changes in antioxidant capacity and formation of reactive oxygen and reactive nitrogen species, we hypothesized that COPD has a similar effect on the diaphragm, which is related to the severity of COPD. Catalase activity was determined spectrophotometrically. Levels of 4-hydroxy-2-nonenal (HNE)-protein adducts and 3-nitrotyrosine (NT) formation were measured using western blotting. Levels of malondialdehyde (MDA) were assessed by high-performance liquid chromatography. We found that catalase activity was approximately 89% higher in the diaphragm of severe COPD patients (FEV1 37+/-5% predicted…

Pulmonary and Respiratory Medicinemedicine.medical_specialtyrespiratory musclesNitrosationDiaphragmOxidative phosphorylationmedicine.disease_causeSeverity of Illness IndexNOLipid peroxidationPulmonary Disease Chronic Obstructivechemistry.chemical_compoundInternal medicineHumansCOPDMedicineReactive nitrogen speciesOriginal ResearchCOPDoxidantsbiologybusiness.industryHealth PolicyPublic Health Environmental and Occupational HealthGeneral MedicineMiddle Agedmedicine.diseaseMalondialdehydeDiaphragm (structural system)Surgeryrespiratory tract diseasesOxidative StressantioxidantsEndocrinologychemistryCatalasebiology.proteinbusinessOxidative stressInternational journal of chronic obstructive pulmonary disease
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Hypoxia-induced dysfunction of rat diaphragm

2004

Contains fulltext : 47331.pdf (Publisher’s version ) (Closed access) Oxidants may play a role in hypoxia-induced respiratory muscle dysfunction. In the present study we hypothesized that hypoxia-induced impairment in diaphragm contractility is associated with elevated peroxynitrite generation. In addition, we hypothesized that strenuous contractility of the diaphragm increases peroxynitrite formation. In vitro force-frequency relationship, isotonic fatigability, and nitrotyrosine levels were assessed under hypoxic (Po(2) approximately 6.5 kPa) and hyperoxic (Po(2) approximately 88.2 kPa) control conditions and also in the presence of authentic peroxynitrite (60 min), ebselen (60 min), and t…

Pulmonary and Respiratory MedicineAzolesMalemedicine.medical_specialtyPhysiologyDiaphragmAetiology screening and detection [ONCOL 5]In Vitro TechniquesIsoindolesNitric oxideContractilitychemistry.chemical_compoundTranslational research [ONCOL 3]Physiology (medical)Internal medicineOrganoselenium CompoundsPeroxynitrous AcidmedicineRespiratory muscleAnimalsRespiratory systemEnzyme InhibitorsRats WistarHypoxiaHeart lung and circulation [UMCN 2.1]Renal disorder [IGMD 9]omega-N-MethylarginineNitrotyrosineCell BiologyHypoxia (medical)Tissue engineering and pathology [NCMLS 3]musculoskeletal systemRatsPathogenesis and modulation of inflammation [N4i 1]EndocrinologychemistryBiochemistryMuscle FatigueTyrosineRat DiaphragmLipid Peroxidationmedicine.symptomPeroxynitriteMuscle ContractionAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
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Xanthine oxidase is involved in exercise-induced oxidative stress in chronic obstructive pulmonary disease

1999

In the present study, we hypothesized that exhaustive exercise in patients with chronic obstructive pulmonary disease (COPD) results in glutathione oxidation and lipid peroxidation and that xanthine oxidase (XO) contributes to free radical generation during exercise. COPD patients performed incremental cycle ergometry until exhaustion with (n = 8) or without (n = 8) prior treatment with allopurinol, an XO inhibitor. Reduced (GSH) and oxidized glutathione (GSSG) and lipid peroxides [malondialdehyde (MDA)] were measured in arterial blood. In nontreated COPD patients, maximal exercise (approximately 75 W) resulted in a significant increase in the GSSG-to-GSH ratio (4. 6 +/- 0.9% at rest vs. 9.…

MaleXanthine OxidasePhysiologyAllopurinolRestPhysical ExertionPhysical exercisePharmacologymedicine.disease_causeLipid peroxidationchemistry.chemical_compoundAdenosine TriphosphatePhysiology (medical)MalondialdehydemedicineHumansLung Diseases ObstructiveXanthine oxidaseCOPDGlutathione DisulfideRespiratory diseaseGlutathioneMiddle Agedmedicine.diseaseGlutathionePathophysiologyOxidative StressBiochemistrychemistryExercise TestFemaleLipid PeroxidationOxidative stress
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