Author: Pascale Seddas-dozolme

0000000000279856

AUTHOR

Pascale Seddas-dozolme

Deciphering the molecular dialogue between arbuscular mycorrhizal partners during early root interactions

International audience

Production of monoclonal antibodies to characterize the tonoplastic polypeptide bob tip 106 of cauliflower cells (brassica oleracea l. Var. Botrytis)

Une banque d'anticorps monoclonaux specifiquement diriges contre des epitopes du tonoplaste des cellules en differenciation du meristeme inflorescentiel de chou-fleur (brassica oleracea l. Var. Botrytis) a ete construite. L'anticorps monoclonal tem 106 qui est specifiquement dirige contre un polypeptide majoritaire (12%) du tonoplaste a ete selectionne. Le polypeptide reconnu par tem 106 a ete appele bob tip 106 pour signifier qu'il s'agit d'un polypeptide de 106 000 daltons intrinseque du tonoplaste de brassica oleracea l. Var. Botrytis. Bob tip 106 est glycosyle et contient des residus mannose ou glucose mais l'epitope est peptidique. Par immuno-microscopie, nous avons montre que bob tip …

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PEPhysical Sciences and Engineering
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