Alternating current voltammetric determination of DNA damage

Abstract The conditions for alternating current (a.c.) voltammetric DNA determinations have been investigated with respect to its use with alkaline filter elution techniques at low DNA concentrations. In inorganic electrolyte solutions three current peaks can be distinguished: peak I around −1.1 V caused by the reorientation or desorption of DNA segments; peak II around −1.2 V caused by the native DNA (nDNA) form; peak III caused by denatured DNA (dDNA) at −1.4 V. Sonication of nDNA increases the peak current, however not with dDNA. Both dDNA and nDNA give linear peak current increments with DNA increments, their regression lines cutting the concentration axis at the origin. In filter eluti…