In vitro effects of extracts from leaves and rhizomes of P.oceanica on HepG2 tumor cells

EVALUATION OF OOCYTE QUALITY IN GRANULOSA AND CUMULUS CELLS OF PATIENTS UNDERGOING PMA

Background. We investigate the apoptosis rate of individual granulosa cell–oocyte and cumulus cell–oocyte (COC), associated with the levels of molecules playing a critical role in the regulation of cell death or survival. These molecular analyses have been done to verify the difference of competence between oocytes producing embryos able to reach the blastocyst stage compared with embryos arrested during the in vitro culture. Methods. From each single follicle: granulosa cells were processed for Western blotting analyses, using the following antibodies: pAKT, ERK 1/2, pERK 1/2; cumulus cells were used for in situ immunofluorescence with the same antibodies. DNA fragmentation rate was measur…

CYTOTOXIC POTENTIAL OF AQUEOUS EXTRACTS FROM RHIZOMES AND LEAVES OF POSIDONIA OCEANICA (L.) DELILE AGAINST HEPG2 CANCER CELLS