OXYGENATION AND BIOENERGETIC STATUS OF HUMAN TUMORS

Abstract of the 68th Meeting (Spring Meeting) 6–9 March 1990, Heidelberg

Geographical mapping of metabolites in biological tissue with quantitative bioluminescence and single photon imaging

This article features a novel technique for measuring the spatial distribution of metabolites, such as ATP, glucose, and lactate, in rapidly frozen tissue. Concentration values are obtained in absolute terms and with a spatial resolution of single-cell dimension. The method is based on enzymatic reactions that link the metabolite of interest to luciferase with subsequent light emission. Using a specific array, cryosections are brought into contact with the enzymes in a well-defined, reproducible way inducing a distribution of light across the section with an intensity that is proportional to the metabolite concentration. The emitted light can be visualized through a microscope and an imagin…

ASSOCIATION OF RAS-TRANSFECTION WITH CHANGES IN FIBROBLAST MORPHOLOGY AND OXYGEN CONSUMPTION