0000000000297598

AUTHOR

Pascual Villalba-bermell

showing 3 related works from this author

Exploring the miRNA-mediated response to combined stresses in melon plants

2021

AbstractClimate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop-productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of melon’s response to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or …

Plant developmentMelonmicroRNADifferentially expressed mirnasComputational biologyBiologyGeneTranscription factorCrop productivity
researchProduct

Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network

2021

Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of response of melon to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple …

MelonBiotic and abiotic stressRNA regulatory networksPlant cultureDifferentially expressed mirnasComputational biologyPlant ScienceBiologyCrop productivitybiotic and abiotic stressSB1-1110crop production and climate changePlant developmentGENETICAMiRNAs and stress response in Cucumis melomiRNAs and stress response in Cucumis melomicroRNACrop production and climate changeRNA regulatory networks; RNA-seq and systems biology; biotic and abiotic stress; crop production and climate change; miRNAs and stress response in Cucumis meloStress conditionsTranscription factorGeneRNA-seq and systems biologyOriginal ResearchFrontiers in Plant Science
researchProduct

Assembly methods for nanopore-based metagenomic sequencing: a comparative study

2019

ABSTRACTBackgroundMetagenomic sequencing has lead to the recovery of previously unexplored microbial genomes. In this sense, short-reads sequencing platforms often result in highly fragmented metagenomes, thus complicating downstream analyses. Third generation sequencing technologies, such as MinION, could lead to more contiguous assemblies due to their ability to generate long reads. Nevertheless, there is a lack of studies evaluating the suitability of the available assembly tools for this new type of data.FindingsWe benchmarked the ability of different short-reads and long-reads tools to assembly two different commercially available mock communities, and observed remarkable differences i…

NanoporeContigMicrobial GenomesComputer scienceMetagenomicsMinionNanopore sequencingComputational biologyBacterial genome sizeGenomeGene
researchProduct