0000000000297599

AUTHOR

María-carmen Marques

showing 3 related works from this author

Exploring the miRNA-mediated response to combined stresses in melon plants

2021

AbstractClimate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop-productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of melon’s response to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or …

Plant developmentMelonmicroRNADifferentially expressed mirnasComputational biologyBiologyGeneTranscription factorCrop productivity
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Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network

2021

Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of response of melon to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple …

MelonBiotic and abiotic stressRNA regulatory networksPlant cultureDifferentially expressed mirnasComputational biologyPlant ScienceBiologyCrop productivitybiotic and abiotic stressSB1-1110crop production and climate changePlant developmentGENETICAMiRNAs and stress response in Cucumis melomiRNAs and stress response in Cucumis melomicroRNACrop production and climate changeRNA regulatory networks; RNA-seq and systems biology; biotic and abiotic stress; crop production and climate change; miRNAs and stress response in Cucumis meloStress conditionsTranscription factorGeneRNA-seq and systems biologyOriginal ResearchFrontiers in Plant Science
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CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation

2021

The novel respiratory virus SARS-CoV-2 is rapidly evolving across the world with the potential of increasing its transmission and the induced disease. Here, we applied the CRISPR-Cas12a system to detect, without the need of sequencing, SARS-CoV-2 genomes harboring the E484K mutation, first identified in the Beta variant and catalogued as an escape mutation. The E484K mutation creates a canonical protospacer adjacent motif for Cas12a recognition in the resulting DNA amplicon, which was exploited to obtain a differential readout. We analyzed a series of fecal samples from hospitalized patients in Valencia (Spain), finding one infection with SARS-CoV-2 harboring the E484K mutation, which was t…

PolymersBiomedical EngineeringBiosensing TechniquesBiologyBiochemistry Genetics and Molecular Biology (miscellaneous)Genomechemistry.chemical_compoundCOVID-19 TestingPeptide LibraryTechnical NoteCRISPRCRISPR diagnosticsHumansGeneticsvirus evolutionSARS-CoV-2Epidemiological surveillanceepidemiological surveillanceCOVID-19General MedicineDNAAmpliconSurface Plasmon ResonanceVirus evolutionProtospacer adjacent motifHEK293 CellschemistryGenetic TechniquesSpainViral evolutionImmunoglobulin GMutation (genetic algorithm)DNA ViralMutationRespiratory virusCRISPR-Cas SystemsDNAACS Synthetic Biology
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