0000000000298108

AUTHOR

Laura Lindholm

showing 3 related works from this author

Molecular and structural characterization of fluorescent human parvovirus B19 virus-like particles

2005

Although sharing a T = 1 icosahedral symmetry with other members of the Parvoviridae family, it has been suggested that the fivefold channel of the human parvovirus B19 VP2 capsids is closed at its outside end. To investigate the possibility of placing a relatively large protein moiety at this site of B19, fluorescent virus-like particles (fVLPs) of B19 were developed. The enhanced green fluorescent protein (EGFP) was inserted at the N-terminus of the structural protein VP2 and assembly of fVLPs from this fusion protein was obtained. Electron microscopy revealed that these fluorescent protein complexes were very similar in size when compared to wild-type B19 virus. Further, fluorescence cor…

Models MolecularImmunoprecipitationRecombinant Fusion ProteinsvirusesGreen Fluorescent ProteinsBiophysicsFluorescence correlation spectroscopyEndosomesSpodopteraBiologyMicroscopy Atomic ForceBiochemistryFluorescenceCell LineGreen fluorescent proteinParvoviridae InfectionsBimolecular fluorescence complementationCell Line Tumorhemic and lymphatic diseasesParvovirus B19 HumanAnimalsHumansImmunoprecipitationMolecular BiologyParvoviridaeImmune SeraVirus AssemblyVirionvirus diseasesCell Biologybiology.organism_classificationFusion proteinMolecular biologyNanostructuresCell biologyTransport proteinProtein TransportCapsidCapsid Proteins
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Exploitation of Microtubule Cytoskeleton and Dynein during Parvoviral Traffic toward the Nucleus

2003

ABSTRACT Canine parvovirus (CPV), a model virus for the study of parvoviral entry, enters host cells by receptor-mediated endocytosis, escapes from endosomal vesicles to the cytosol, and then replicates in the nucleus. We examined the role of the microtubule (MT)-mediated cytoplasmic trafficking of viral particles toward the nucleus. Immunofluorescence and immunoelectron microscopy showed that capsids were transported through the cytoplasm into the nucleus after cytoplasmic microinjection but that in the presence of MT-depolymerizing agents, viral capsids were unable to reach the nucleus. The nuclear accumulation of capsids was also reduced by microinjection of an anti-dynein antibody. More…

Parvovirus CaninevirusesImmunoelectron microscopyImmunologyDyneinActive Transport Cell Nucleusmacromolecular substancesMicrotubulesMicrobiologyMotor proteinCapsidCytosolMicrotubuleVirologymedicineAnimalsCytoskeletonCytoskeletonCell NucleusbiologyDyneinsbiochemical phenomena metabolism and nutritionVirus-Cell InteractionsCell biologyMicroscopy ElectronTubulinmedicine.anatomical_structureCytoplasmInsect ScienceCatsbiology.proteinNucleusJournal of Virology
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Resurssierityislastentarhanopettaja lapsen sosioemotionaalisen kehityksen tukijana

2015

Tutkimuksen tavoitteena oli selvittää resurssierityislastentarhanopettajan työnkuvaa lapsen sosioemotionaalisen kehityksen tukijana. Tästä näkökulmasta tutkimuksessa kartoitettiin relton toimenkuvaa ja työn tavoitteita. Tutkimuksen tavoitteena oli selvittää, millaisia sosioemotionaalisia tuen tarpeita lapsilla oli ja miten relto tuki heidän kehitystään päiväkodissa. Lisäksi tutkittiin, miten kolmiportaisen tuen malli näkyi resurssierityislastentarhanopettajan työssä sosioemotionaalista tukea saavan lapsen kanssa ja minkälaista yhteistyötä resurssierityislastentarhanopettaja teki sosioemotionaalista tukea saavan lapsen kanssa toimiessaan. Tutkimus toteutettiin haastattelututkimuksena etelä–s…

tunne-elämän kehitysvarhaiskasvatussosioemotionaalisen kehityksen tuen tarpeetresurssierityislastentarhanopettajaerityiskasvatusvarhaiserityiskasvatuserityislastentarhanopettajat
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