0000000000298108
AUTHOR
Laura Lindholm
Molecular and structural characterization of fluorescent human parvovirus B19 virus-like particles
Although sharing a T = 1 icosahedral symmetry with other members of the Parvoviridae family, it has been suggested that the fivefold channel of the human parvovirus B19 VP2 capsids is closed at its outside end. To investigate the possibility of placing a relatively large protein moiety at this site of B19, fluorescent virus-like particles (fVLPs) of B19 were developed. The enhanced green fluorescent protein (EGFP) was inserted at the N-terminus of the structural protein VP2 and assembly of fVLPs from this fusion protein was obtained. Electron microscopy revealed that these fluorescent protein complexes were very similar in size when compared to wild-type B19 virus. Further, fluorescence cor…
Exploitation of Microtubule Cytoskeleton and Dynein during Parvoviral Traffic toward the Nucleus
ABSTRACT Canine parvovirus (CPV), a model virus for the study of parvoviral entry, enters host cells by receptor-mediated endocytosis, escapes from endosomal vesicles to the cytosol, and then replicates in the nucleus. We examined the role of the microtubule (MT)-mediated cytoplasmic trafficking of viral particles toward the nucleus. Immunofluorescence and immunoelectron microscopy showed that capsids were transported through the cytoplasm into the nucleus after cytoplasmic microinjection but that in the presence of MT-depolymerizing agents, viral capsids were unable to reach the nucleus. The nuclear accumulation of capsids was also reduced by microinjection of an anti-dynein antibody. More…
Resurssierityislastentarhanopettaja lapsen sosioemotionaalisen kehityksen tukijana
Tutkimuksen tavoitteena oli selvittää resurssierityislastentarhanopettajan työnkuvaa lapsen sosioemotionaalisen kehityksen tukijana. Tästä näkökulmasta tutkimuksessa kartoitettiin relton toimenkuvaa ja työn tavoitteita. Tutkimuksen tavoitteena oli selvittää, millaisia sosioemotionaalisia tuen tarpeita lapsilla oli ja miten relto tuki heidän kehitystään päiväkodissa. Lisäksi tutkittiin, miten kolmiportaisen tuen malli näkyi resurssierityislastentarhanopettajan työssä sosioemotionaalista tukea saavan lapsen kanssa ja minkälaista yhteistyötä resurssierityislastentarhanopettaja teki sosioemotionaalista tukea saavan lapsen kanssa toimiessaan. Tutkimus toteutettiin haastattelututkimuksena etelä–s…