0000000000300381

AUTHOR

L. Ranjard

showing 2 related works from this author

Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: Biological and Methodologic…

2001

ABSTRACT Automated rRNA intergenic spacer analysis (ARISA) was used to characterise bacterial (B-ARISA) and fungal (F-ARISA) communities from different soil types. The 16S-23S intergenic spacer region from the bacterial rRNA operon was amplified from total soil community DNA for B-ARISA. Similarly, the two internal transcribed spacers and the 5.8S rRNA gene (ITS1-5.8S-ITS2) from the fungal rRNA operon were amplified from total soil community DNA for F-ARISA. Universal fluorescence-labeled primers were used for the PCRs, and fragments of between 200 and 1,200 bp were resolved on denaturing polyacrylamide gels by use of an automated sequencer with laser detection. Methodological (DNA extracti…

DNA BacterialRibosomal Intergenic Spacer analysisBiologyPolymerase Chain ReactionApplied Microbiology and Biotechnology03 medical and health sciencesIntergenic regionRNA Ribosomal 16SDNA Ribosomal SpacerMethodsDNA FungalComputingMilieux_MISCELLANEOUSEcosystemSoil Microbiology030304 developmental biology[SDV.EE]Life Sciences [q-bio]/Ecology environmentGenetics[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology0303 health sciencesBacteriaEcology030306 microbiologyFungiReproducibility of ResultsGenes rRNASpacer DNABIOLOGIE MOLECULAIRERibosomal RNADNA FingerprintingDNA extraction[SDV.EE] Life Sciences [q-bio]/Ecology environmentRNA Ribosomal 23SDNA profilingRRNA Operon[SDE.BE]Environmental Sciences/Biodiversity and EcologySoil microbiologyFood ScienceBiotechnologyApplied and Environmental Microbiology
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Relevance of different soil fauna and microflora groups in the monitoring of soil biodiversity: RMQS-Biodiv, a French pilot area experience.

2008

PosterFR2116Poster

[SDE.MCG] Environmental Sciences/Global Changes[ SDE.MCG ] Environmental Sciences/Global Changes[SDE.MCG]Environmental Sciences/Global Changes[ SDU.ENVI ] Sciences of the Universe [physics]/Continental interfaces environmentComputingMethodologies_GENERAL[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces environment
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