Early life exposure to estrogen-mimics increase the occurrence of uterine fibroids via expansion of myometrial stem cell population

COMPARISON OF OPERATIVE AND PERI-OPERATIVE RESULTS FOR ROBOTIC AND LAPAROSCOPIC MYOMECTOMIES. C. E. Miller, K. Sasaki, C. Steller, S. Sulo, A. Cholkeri-Singh. The Advanced IVF Institute, Naperville, IL; Advanced Gynecologic Surgery Institute, Naperville, IL; Advocate Lutheran General Hospital, Park Ridge, IL; Center for Advanced Care, Russell Institute of Research and Innovation, Park Ridge, IL; The Advanced Gynecologic Surgery Institute, Naperville, IL.

Role of Stro1+/CD44+ stem cells in myometrial physiology and uterine remodeling during pregnancy†

Regulation of myometrial functions during pregnancy has been considered the result of the integration of endocrine and mechanical signals. Nevertheless, uterine regeneration is poorly understood, and the cellular source within the gravid uterus is largely unexplored.In this study, we isolated and quantified the myometrial stem cells (MSC) population from pregnant female Eker rat uteri, by using Stro1/CD44 surface markers. We demonstrated that prior parity significantly increased the percentage of Stro1+/CD44+ MSC because of injured tissue response. Interestingly, we established that Stro1+/CD44+ MSC respond efficiently to physiological cues when they were treated in vitro under different do…

Synergistic effect of estrogen and progesterone on myometrial stem cell expansion in vivo

Stro-1/CD44 as putative human myometrial and fibroid stem cell markers

Objective To identify and characterize myometrial/fibroid stem cells by specific stem cell markers in human myometrium, and to better understand the stem cell contribution in the development of uterine fibroids. Design Prospective, experimental human and animal study. Setting University research laboratory. Patient(s)/Animal(s) Women undergoing hysterectomy for treatment of symptomatic uterine fibroids and female NOD/SCID/IL-2Rγ null mice. Intervention(s) Identification and isolation of stem cells from human fibroids and adjacent myometrium tissues using Stro-1/CD44–specific surface markers. Main Outcome Measure(s) Flow cytometry, semiquantitative polymerase chain reaction, clonogenicity as…