0000000000305327

AUTHOR

V Navarro

showing 2 related works from this author

Meningitis due to Pseudomonas stutzeri in a patient infected with human immunodeficiency virus.

1996

Microbiology (medical)AdultMalebiologyAIDS-Related Opportunistic Infectionsbusiness.industrybiology.organism_classificationmedicine.diseaseVirologyMicrobiologyPseudomonas stutzeriMeningitis BacterialInfectious DiseasesAcquired immunodeficiency syndrome (AIDS)ImmunopathologyPseudomonasPseudomonadalesMedicineHumansPseudomonas InfectionsViral diseasebusinessSidaMeningitisPseudomonadaceaeClinical infectious diseases : an official publication of the Infectious Diseases Society of America
researchProduct

DNA delivery to 'ex vivo' human liver segments.

2011

Hydrodynamic injection is an efficient procedure for liver gene therapy in rodents but with limited efficacy in large animals, using an 'in vivo' adapted regional hydrodynamic gene delivery system. We study the ability of this procedure to mediate gene delivery in human liver segments obtained by surgical resection. Watertight liver segments were retrogradely injected from hepatic vein with a saline solution containing a plasmid bearing the enhanced green fluorescent protein (eGFP) gene, under different conditions of flow rate (1, 10 and 20 ml s(-1)) and final perfused volume. Samples were cultured for 1 to 2 days and used for microscopy and molecular analysis of gene expression. The fluore…

Genetic enhancementGreen Fluorescent ProteinsGene Transfer TechniquesGenetic TherapyBiologyGene deliveryHepatic VeinsMolecular biologyGreen fluorescent proteinCatheterizationLiverIn vivoTranscription (biology)Gene expressionInjections IntravenousGeneticsHydrodynamicsMolecular MedicineHumansMolecular BiologyGeneEx vivoPlasmidsGene therapy
researchProduct