0000000000312131

AUTHOR

Kristina Rathsack

showing 2 related works from this author

In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice

2006

SummaryMale gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells. These cells are able to undergo meiosis, are able to generate haploid male gametes in vitro, and are functional, as shown by fertilization after intracytoplasmic injection into mouse oocytes. Resulting two-cell embryos were transferred into oviducts, and live mice were born. Six of seven animals developed to adult mice. This is a clear indication that male gametes derived in vitro from ES cells by this…

MaleGreen Fluorescent ProteinsPopulationDNA RecombinantDEVBIOMice TransgenicIn Vitro TechniquesBiologyGeneral Biochemistry Genetics and Molecular BiologyCell LineMice03 medical and health sciences0302 clinical medicinePregnancymedicineAnimalsSperm Injections IntracytoplasmicSpermatogenesiseducationMolecular BiologyGametogenesis030304 developmental biology0303 health scienceseducation.field_of_study030219 obstetrics & reproductive medicineBase SequenceStem CellsCell DifferentiationEmbryoCell BiologyEmbryo TransferSTEMCELLEmbryonic stem cellRecombinant ProteinsSpermatogoniaCell biologyLuminescent ProteinsMeiosismedicine.anatomical_structureImmunologyGameteFemalePloidyStem cellSpermatogenesisStem Cell TransplantationDevelopmental BiologyDevelopmental Cell
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Männliche Keimzellen aus embryonalen Stammzellen

2007

Zusammenfassung Die Grundlage für die lebenslange Differenzierung männlicher Keimzellen sind die spermatogonialen Stammzellen (SSC) im Testis (etwa 0,03% aller Testiszellen). Es ist gelungen, aus embryonalen Stammzellen (ES-Zellen) der Maus SSC zu generieren, die die Meiose und die Haploidisierung durchlaufen. Werden die entstehenden Spermien mit Hilfe von ICSI in unbefruchtete Eizellen eingebracht und die entstandenen 2-Zeller in den Uterus pseudoschwangerer Mäuse transferiert, werden lebensfähige Mäuse geboren. Die von uns entwickelte Strategie ermöglicht molekulare und biochemische Untersuchungen zur männlichen Keimzelldifferenzierung, insbesondere auch zur Meiose und zur Haploidisierung.

2. Zero hunger0303 health sciences03 medical and health sciences0302 clinical medicineGenetics030217 neurology & neurosurgeryGenetics (clinical)3. Good health030304 developmental biologyMedizinische Genetik
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