0000000000315656
AUTHOR
O. Moukadiri
Physiological and genomic variations in rice cells recovered from direct immersion and storage in liquid nitrogen
The use of cryoprotectants and slow cooling rates are routine procedures for the cryopreservation of plant cell lines. However, our results with rice (Oryza sativa L., cv. Taipei 309) show that calli can be cryopreserved by direct immersion and stored in liquid nitrogen without any cryoprotection. The efficiency of recovery using this method, as well as a conventional method was generally increased with a previous abscisic acid (ABA) treatment. Following cryopreservation, calli demonstrated some differences with respect to unfrozen calli of the same lines. Thus, resistance to freezing stress (−20°C for 2 h) increased significantly in all lines tested, irrespective of their pre-incubation wi…
Phenotypic characterization of the progenies of rice plants derived from cryopreserved calli
The progenies of rice plants (Oryza sativa L.) differentiated from calli that had been cryopreserved and from control (non-cryopreserved) calli were used to study the influence of selection pressure during cryopreservation. The phenotypic evaluation of these progenies was based mainly on the response of seedlings and calli to freezing stress and on the characterization of protoplast and cell populations by flow cytometric analyses. The patterns of response to freezing stress, as well as the variations in some morphological and physiological cell parameters, were unrelated to the origin (cryopreserved or control calli) of the parental plants.