0000000000324718

AUTHOR

Theo Thijs

showing 2 related works from this author

Chemosensory signalling pathways involved in sensing of amino acids by the ghrelin cell

2015

AbstractTaste receptors on enteroendocrine cells sense nutrients and transmit signals that control gut hormone release. This study aimed to investigate the amino acid (AA) sensing mechanisms of the ghrelin cell in a gastric ghrelinoma cell line, tissue segments and mice. Peptone and specific classes of amino acids stimulate ghrelin secretion in the ghrelinoma cell line. Sensing of L-Phe occurs via the CaSR, monosodium glutamate via the TAS1R1-TAS1R3 while L-Ala and peptone act via 2 different amino acid taste receptors: CaSR & TAS1R1-TAS1R3 and CaSR & GPRC6A, respectively. The stimulatory effect of peptone on ghrelin release was mimicked ex vivo in gastric but not in jejunal tissue …

medicine.medical_specialty[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionEnteroendocrine cellGPRC6ANutrient sensingBiologyArticleReceptors G-Protein-CoupledMice03 medical and health sciences0302 clinical medicineGlucagon-Like Peptide 1Receptor-Interacting Protein Serine-Threonine Kinase 2Taste receptorCell Line TumorInternal medicinemedicineFood and NutritionAnimalsAmino AcidsReceptor030304 developmental biology0303 health sciencesMultidisciplinarydigestive oral and skin physiologyGhrelinEndocrinologySomatostatinReceptor-Interacting Protein Serine-Threonine KinasesAlimentation et NutritionGhrelin[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition030217 neurology & neurosurgeryGhrelin secretionhormones hormone substitutes and hormone antagonistsSignal TransductionScientific Reports
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Smooth muscle and neural dysfunction contribute to different phases of murine postoperative ileus.

2015

Background Postoperative ileus (POI) is characterized by a transient inhibition of gastrointestinal (GI) motility after abdominal surgery mediated by the inflammation of the muscularis externa (ME). The aim of this study was to identify alterations in the enteric nervous system that may contribute to the pathogenesis of POI. Methods Gastrointestinal transit, contractility of isolated smooth muscle strips and inflammatory parameters were evaluated at different time points (1.5 h to 10 days) after intestinal manipulation (IM) in mice. Immune-labeling was used to visualize changes in myenteric neurons. Key Results Intestinal manipulation resulted in an immediate inhibition of GI transit recove…

0301 basic medicinemedicine.medical_specialtyCarbacholIleusPhysiologyInflammationBiologyEnteric Nervous SystemProinflammatory cytokineContractility03 medical and health sciencesMiceIleusOrgan Culture TechniquesPostoperative ComplicationsInternal medicinemedicineAnimalsEndocrine and Autonomic SystemsGastroenterologyMuscle SmoothSmooth muscle contractionmedicine.diseaseMice Inbred C57BL030104 developmental biologyEndocrinologyAnesthesiaCholinergicEnteric nervous systemFemalemedicine.symptomInflammation MediatorsGastrointestinal Motilitymedicine.drugNeurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society
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