0000000000329700

AUTHOR

Sabine Hunke

showing 2 related works from this author

The Sensor Kinase DctS Forms a Tripartite Sensor Unit with DctB and DctA for Sensing C4-Dicarboxylates in Bacillus subtilis

2013

The DctSR two-component system of Bacillus subtilis controls the expression of the aerobic C4-dicarboxylate transporter DctA. Deletion of DctA leads to an increased dctA expression. The inactivation of DctB, an extracellular binding protein, is known to inhibit the expression of dctA. Here, interaction between the sensor kinase DctS and the transporter DctA as well as the binding protein DctB was demonstrated in vivo using streptavidin (Strep) or His protein interaction experiments (mSPINE or mHPINE), and the data suggest that DctA and DctB act as cosensors for DctS. The interaction between DctS and DctB was also confirmed by the bacterial two-hybrid system (BACTH). In contrast, no indicati…

StreptavidinRegulation of gene expressionKinaseBinding proteinMembrane ProteinsTransporterGene Expression Regulation BacterialArticlesPlasma protein bindingBacillus subtilisBiologybiology.organism_classificationMicrobiologyGene Expression Regulation Enzymologicchemistry.chemical_compoundPlasmidBacterial ProteinsBiochemistrychemistryDicarboxylic AcidsCarrier ProteinsMolecular BiologyBacillus subtilisPlasmidsProtein BindingJournal of Bacteriology
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The Escherichia coli Envelope Stress Sensor CpxA Responds to Changes in Lipid Bilayer Properties

2015

The Cpx stress response system is induced by various environmental and cellular stimuli. It is also activated in Escherichia coli strains lacking the major phospholipid, phosphatidylethanolamine (PE). However, it is not known whether CpxA directly senses changes in the lipid bilayer or the presence of misfolded proteins due to the lack of PE in their membranes. To address this question, we used an in vitro reconstitution system and vesicles with different lipid compositions to track modulations in the activity of CpxA in different lipid bilayers. Moreover, the Cpx response was validated in vivo by monitoring expression of a PcpxP-gfp reporter in lipid-engineered strains of E. coli. Our comb…

Models MolecularCardiolipinsSurface PropertiesRecombinant Fusion ProteinsGreen Fluorescent ProteinsLipid BilayersArabidopsisPhospholipidBiologymedicine.disease_causeBiochemistrychemistry.chemical_compoundBacterial ProteinsGenes ReportermedicineAcholeplasma laidlawiiPhosphorylationLipid bilayerEscherichia coliPlant ProteinsPhosphatidylethanolamineEscherichia coli ProteinsPhosphatidylethanolaminesVesicleGlycosyltransferasesMembrane ProteinsPhosphatidylglycerolsCell biologychemistryMembrane proteinlipids (amino acids peptides and proteins)Protein foldingSignal transductionProtein KinasesProtein Processing Post-TranslationalSignal TransductionBiochemistry
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