Irreversible binding of acrylonitrile to nucleic acids

1. [2,3-14C]Acrylonitrile was incubated with rat-liver microsomes, NADPH and either DNA, RNA or bovine serum albumin. Irreversible binding occurred to the macromolecular targets. Binding was lower when incubations were performed without microsomes. 2. Most of the 14C bound to DNA, RNA or polynucleotides (poly-A, poly-C, poly-G, poly-U) after incubation of [2,3-14C]acrylonitrile with rat-liver microsomes and 'conventional' re-isolation of the nucleic acids was removed from the macromolecular target when subsequently chromatographed on hydroxyapatite. 3. Radioactivity attached to DNA after prolonged non-enzymic incubations with [2,3-14C]acrylonitrile was also removed from the DNA by chromatog…

Hypophysäre/Ovarielle Wirkstoffe

Measurement of W ± Z production in proton-proton collisions at √s =7 TeV with the ATLAS detector

A study of W±Z production in proton-proton collisions at √s=7 TeV is presented using data corresponding to an integrated luminosity of 4.6 fb−1 collected with the ATLAS detector at the Large Hadron Collider in 2011. In total, 317 candidates, with a background expectation of 68±10 events, are observed in double-leptonic decay final states with electrons, muons and missing transverse momentum. The total cross-section is determined to be σtotWZ=19.0+1.4−1.3(stat.)±0.9(syst.)±0.4(lumi.) pb, consistent with the Standard Model expectation of 17.6+1.1−1.0 pb. Limits on anomalous triple gauge boson couplings are derived using the transverse momentum spectrum of Z bosons in the selected events. The …