0000000000349719

AUTHOR

Mercedes Dávila

showing 2 related works from this author

EVOLUTIONARY DYNAMICS OF FITNESS RECOVERY FROM THE DEBILITATING EFFECTS OF MULLER'S RATCHET.

1998

The great adaptability shown by RNA viruses is a consequence of their high mutation rates. The evolution of fitness in a severely debilitated, clonal population of the nonsegmented ribovirus vesicular stomatitis virus (VSV) has been compared under five different demographic regimes, ranging from severe serial bottleneck passages (one virion) to large population passages (105 virions or more) under similar environmental conditions (cell culture type and temperature). No matter how small the bottleneck, the fitness of the evolved populations was always higher than the fitness of the starting population; this result is clearly different from that previously reported for viruses with higher fit…

0106 biological sciences0301 basic medicineGeneticsExperimental evolutionMutation rateeducation.field_of_studybiologyvirusesPopulationMuller's ratchetbiology.organism_classification010603 evolutionary biology01 natural sciencesVirus03 medical and health sciences030104 developmental biologyVesicular stomatitis virusGeneticsAdaptationGeneral Agricultural and Biological SciencesEvolutionary dynamicseducationEcology Evolution Behavior and SystematicsEvolution; international journal of organic evolution
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Genetic lesions associated with Muller's ratchet in an RNA virus

1996

The molecular basis of Muller's ratchet has been investigated using the important animal pathogen foot-and-mouth disease virus (FMDV). Clones from two FMDV populations were subjected to serial plaque transfers (repeated bottleneck events) on host BHK-21 cells. Relative fitness losses were documented in 11 out of 19 clones tested. Small fitness gains were observed in three clones. One viral clone attained an extremely low plating efficiency, suggesting that accumulation of deleterious mutations had driven the virus near extinction. Nucleotide sequence analysis revealed unique genetic lesions in multiply transferred clones that had never been seen in FMDVs isolated in nature or subjected to m…

Mutational hot spotvirusesViral quasispeciesViral Plaque AssayVirusOligoadenylate extensionCell LineExtinction PsychologicalAphthovirusStructural BiologyCricetinaePolyadenylateAnimalsFitness lossMolecular BiologyGeneticsbiologyFoot-and-mouth disease virusNucleic acid sequenceRNA virusMuller's ratchetbiology.organism_classificationVirologyQuasispeciesCapsidMutationNucleic Acid ConformationRNA ViralFoot-and-mouth disease virus
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