0000000000350026

AUTHOR

Arianna Troilo

showing 1 related works from this author

Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis

2018

Background Tuberculosis (TB) still is a major worldwide health problem, with 10.4 million new cases in 2016. Only 5–15% of people infected with M. tuberculosis develop TB disease while others remain latently infected (LTBI) during their lifetime. Thus, the absence of tests able to distinguish between latent infection and active tuberculosis is one of the major limits of currently available diagnostic tools. Methods A total of 215 patients were included in the study as active TB cases (n = 73), LTBI subjects (n = 88) and healthy persons (n = 54). Peripheral blood mononuclear cells (PBMCs) were isolated from each patient and the LIOSpot® TB anti-human IL-2 ELISpot assay was performed to test …

Bacterial DiseasesMale0301 basic medicinelcsh:MedicineAdult; Aged; Case-Control Studies; Diagnosis Differential; Female; Humans; Immunoassay; Interleukin-2; Latent Tuberculosis; Male; Middle Aged; Mycobacterium tuberculosis; ROC Curve; Species SpecificityFluorescence MicroscopyBiochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)ZoonosesDiagnosisMedicine and Health SciencesBovine TuberculosisEnzyme-Linked Immunoassayslcsh:ScienceImmunoassayMicroscopyMultidisciplinarybiologyLatent tuberculosismedicine.diagnostic_testELISPOTLight MicroscopyMiddle AgedActinobacteriaInfectious DiseasesTuberculosis Diagnosis and ManagementFemaleResearch ArticleAdultTuberculosis030106 microbiologyResearch and Analysis MethodsQuantiFERONDiagnosis DifferentialMycobacterium tuberculosis03 medical and health sciencesSpecies SpecificityAntigenDiagnostic MedicineLatent TuberculosismedicineTuberculosisHumansImmunoassaysAgedBacteriabusiness.industrylcsh:ROrganismsCase-control studyBiology and Life SciencesMycobacterium tuberculosisTropical Diseasesmedicine.diseasebiology.organism_classificationMycobacterium Ulcerans030104 developmental biologyROC CurveCase-Control StudiesImmunoassayDifferentialImmunologyImmunologic TechniquesInterleukin-2lcsh:QbusinessPLOS ONE
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