0000000000352068
AUTHOR
Guillaume Arnaud
Epidémiologie moléculaire et diversité des phytoplasmes du groupe 16SrV présents dans les compartiments viticoles et sauvages en Europe
National audience
Sequence typing of map, secY and degV genes confirm the genetic relatedness of three distinct groups of flavescence dorée phytoplasmas with phytoplasmas of the 16SrV group infecting grapevine and alder in Europe
International audience
Multilocus sequence typing confirms the close genetic inter-relatedness between three distinct flavescence doree phytoplasma strain clusters and group 16SrV phytoplasmas infecting grapevine and alder in Europe
ABSTRACT Vineyards of southern France and northern Italy are affected by the flavescence dorée (FD) phytoplasma, a quarantine pathogen transmitted by the leafhopper of Nearctic origin Scaphoideus titanus . To better trace propagation of FD strains and identify possible passage between the vineyard and wild plant compartments, molecular typing of phytoplasma strains was applied. The sequences of the two genetic loci map and uvrB - degV , along with the sequence of the secY gene, were determined among a collection of FD and FD-related phytoplasmas infecting grapevine, alder, elm, blackberry, and Spanish broom in Europe. Sequence comparisons and phylogenetic analyses consistently indicated the…
A chromosome map of the Flavescence dorée phytoplasma
International audience; The Flavescence dorée phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were perfor…
Progress towards a chromosome map of the “flavescence dorée” phytoplasma
International audience
Map gene as a new non ribosomal marker to study the molecular variability of grapevine yellows and related phytoplasmas from group 16SRV in Europe
International audience