0000000000353532
AUTHOR
Nathalie Cochennec-laureau
Pmarg-pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera.
11 pages; International audience; The shell of pearl oysters is organized in multiple layers of CaCO(3) crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More s…
Additional file 1 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 1:Table S1: Summary of BlastX results of biomineralization-related protein in the EST P. margaritifera mantle database. A catalogue of 82 P. margaritifera mantle transcripts potentially implicated in the biomineralization process was constructed using BlastX (E-value < 10-3) with selected protein sequences identified from mollusks (bivalvia and gastropoda). (DOC 140 KB)
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…
Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Authors’ original file for figure 1