0000000000353534
AUTHOR
David Piquemal
Pmarg-pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera.
11 pages; International audience; The shell of pearl oysters is organized in multiple layers of CaCO(3) crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More s…
Additional file 1 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 1:Table S1: Summary of BlastX results of biomineralization-related protein in the EST P. margaritifera mantle database. A catalogue of 82 P. margaritifera mantle transcripts potentially implicated in the biomineralization process was constructed using BlastX (E-value < 10-3) with selected protein sequences identified from mollusks (bivalvia and gastropoda). (DOC 140 KB)
Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for the sustainable development of pearl farming industry in French Polynesia.
11 pages; International audience
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 3 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding t…
Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…
'Shellome': Proteins involved in mollusk shell biomineralization - diversity, functions.
18 pages; International audience
Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Authors’ original file for figure 3
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Authors’ original file for figure 1