0000000000361849
AUTHOR
J. Núñez
The effect of scents on the territorial and aggressive behaviour of laboratory rats
The majority of works found in the literature mention odours as an important factor in the development of social lines and the unleashing of aggressive behaviour, but very few authors have studied more deeply the role that these odours play, how they influence behaviour and what importance the variations of these marks of identity may have. In the present work we analyse social relations presented by laboratory rats (Rattus norvegicus, Wistar breed) in seminatural conditions, with special reference to behaviours of dominance, territorialism and aggressiveness, and the importance that these marks of odour play on these behaviours. For this purpose, different individuals (from the established…
Ejaculate fractioning effect on llama sperm head morphometry as assessed by the ISAS(®) CASA system.
Contents South American camelid sperm characteristics are poorly known compared with those of other domestic animals. The long-term duration of ejaculation makes difficult to gather all the seminal fluid, implying possible ejaculation portion losses. Thus, the aim of this research was to evaluate the characteristics of the morphology and morphometry of the spermatozoa change during ejaculation. The morphometric characterization was tested on nine specimens of the Lanuda breed, using a special artificial vagina. In five of the animals, a fractioning of the ejaculate was performed by taking samples every 5 min. for a total of 20 min. Air-dried seminal smears were stained with Hemacolor and mo…
Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish (Danio rerio) spermatozoa.
An increase in reactive oxygen species (ROS) or decrease in antioxidant barriers can provoke lipid peroxidation of the membranes or DNA damage of the spermatozoa. The aim of this work is to study the effect of the different degrees of oxidative stress generated by H2 O2 incubation on total motility, kinetics, and DNA fragmentation of zebrafish (Danio rerio) spermatozoa. For this process, experimental groups were incubated in 50 µM (Low; L) and 200 µM (High; H) H2 O2 , respectively, for 20 min at 4oC. Sperm motility parameters were obtained with a computer-assisted sperm analysis (CASA) system. Sperm DNA fragmentation (SDF) was assessed using the sperm chromatin dispersion test. Both low and…
Facial attractiveness in men provides clues to semen quality
Abstract Facial attractiveness has been related to health in both men and women. Certain psychological, physiological, and secondary sex characteristics have been used as accurate markers of hormonal and developmental health. The main objective of this study was to investigate the capacity of women to select males of high reproductive quality based on their facial attractiveness. A total of 66 males were included in the study. Each of them provides a semen sample, and frontal and lateral photographs were taken. Semen analysis was made according to standard WHO (1999) guidelines for morphology, motility, and concentration. Moreover, a Sperm Index (SI) was calculated as the principal componen…
Early outcomes and complications following cardiac surgery in patients testing positive for coronavirus disease 2019: An international cohort study
The outbreak of severe acute respiratory syndromecoronavirus-2, the cause of coronavirus disease 2019 (COVID-19) in December 2019 represented a global emergency accounting for more than 2.5 million deaths worldwide.1 It has had an unprecedented influence on cardiac surgery internationally, resulting in cautious delivery of surgery and restructuring of services.2 Understanding the influence of COVID-19 on patients after cardiac surgery is based on assumptions from other surgical specialties and single-center studies. The COVIDSurg Collaborative conducted a multicenter cohort study, including 1128 patients, across 235 hospitals, from 24 countries demonstrating perioperative COVID-19 infection…
Effects of exposure to static magnetic fields on the morphology and morphometry of mouse epididymal sperm.
Morphologic and morphometric sperm characteristics of mouse epididymal extracts from animals exposed to static magnetic fields were evaluated. For this purpose, animals were exposed for 35 days to a field of 0.7 T generated by a commercial permanent magnet for either 1 or 24 h per day. The values of morphometric parameters were obtained using the morphometric module of the Sperm Class Analyzer® computerized image analysis system, and percentages of abnormalities were calculated. The size of sperm heads was unaffected by exposure to static magnetic fields. Lack of hook was a sperm head abnormality found significantly more frequently in animals exposed continually than in nonexposed animals, …
Regionalization of epididymal duct and epithelium in rats and mice by automatic computer-aided morphometric analysis.
Aim: To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis. Methods: Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin’s fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 µm and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput (rat and mouse), one (mouse) or three (rat) in the corpus and one in the cauda (rat and mouse). Using the Epiquatre software, several t…
Effect of solid storage at 15 degrees C on the subsequent motility and fertility of rabbit semen.
We conducted two studies to improve preservation of rabbit semen. The objective of the first study was determine whether a glucose- and fructose-based extender with two different amounts of gelatin would solidify at 15 degrees C, and to evaluate the influence of gelatin supplementation on sperm motility parameters after storing semen up to 10 days at 15 degrees C. The fertility of rabbit semen diluted in the best gelatin-supplemented extender established in Study 1 and stored for up to 5 days was evaluated in the second study. In Study 1, semen was collected with an artificial vagina from 40 bucks. Each ejaculate was diluted to (80-100) x 10(6) spermatozoa/mL (1:3, semen/extender) at 37 deg…
A comparison of the effects of bilateral efferent duct ligation and of partial epididymectomy on the testes of rats.
Testis weight as a percentage of body weight did not change following bilateral ligation of the efferent ducts (EDL) close to the epididymis, whereas following removal of part of the epididymis between the site of ligation and a point close to the junction between the caput and corpus (PCE), testis weight first rose linearly until Day 4 and then showed an exponential decrease between Days 4 and 28. After EDL, the perimeter of the seminiferous tubules rose for the first 7 days and then remained elevated, whereas after PCE, there was a linear decrease between Days 4 and 28. Following EDL, the percentage of altered and degenerated tubular cross-sections rose to about 30% and 10%, respectively,…
Male Facial Anthropometry and Attractiveness
The symmetry and masculinity of the face are often considered important elements of male facial attractiveness. However, facial preferences are rarely studied on natural faces. We studied the effect of these traits and facial metric parameters on facial attractiveness in Spanish and Colombian raters. In total, 13 metric and 11 asymmetry parameters from natural, unmanipulated frontal face photographs of 50 Spanish men were measured with the USIA semiautomatic anthropometric software. All raters (women and men) were asked to rank these images as potential long-term partners for females. In both sexes, facial attractiveness was negatively associated with facial masculinity, and preference was …
Brentuximab Vedotin Plus ESHAP (BRESHAP) Versus ESHAP As Salvage Strategy for Patients with Primary Refractory or Relapsed Classical Hodgkin's Lymphoma. Preliminary Results from the Breselibet Prospective Clinical Trial
Abstract Introduction. Patients with relapsed/refractory classical Hodgkin's lymphoma (RRHL) still represent a therapeutic challenge. Consolidation with autologous stem cell transplantation (auto-HCT) is the standard of care in this setting. The achievement of a metabolic complete remission (mCR) with salvage chemotherapy (CT) improves long-term outcome after auto-HCT. The introduction of new drugs has significantly changed the landscape of RRHL. Our cooperative group (GELTAMO) has already demonstrated that brentuximab vedotin (BV) + ESHAP (BRESHAP, García-Sanz R et al, Ann Oncol 2019) is able to achieve a mCR rate of 70% before auto-HCT in patients with RRHL. Nevertheless, the superiority …
Comparison of three different staining methods for the assessment of epididymal red deer sperm morphometry by computerized analysis with ISAS®
When collection of ejaculated sperm samples is not possible, as is the case with wild species, the epididymides of sacrificed wild males become the only possible source of spermatozoa. Mature cauda epididymal spermatozoa display characteristics similar to those of ejaculated sperm cells. The present work proposes a sperm staining technique suitable for the morphometric evaluation of red deer epididymal sperm using a new computerized system. Epididymides from wild animals were extracted no later than 2 h post mortem. After epididymal sectioning, sperm samples were collected, cooled to and equilibrated at 5 °C, and frozen in liquid nitrogen. Before staining, sperm samples were thawed for 20 s…
Male facial attractiveness and masculinity may provide sex- and culture-independent cues to semen quality
Phenotype-linked fertility hypothesis (PLFH) predicts that male secondary sexual traits reveal honest information about male fertilization ability. However, PLFH has rarely been studied in humans. The aim of the present study was to test PLFH in humans and to investigate whether potential ability to select fertile partners is independent of sex or cultural background. We found that on the contrary to the hypothesis, facial masculinity was negatively associated with semen quality. As increased levels of testosterone have been demonstrated to impair sperm production, this finding may indicate a trade-off between investments in secondary sexual signalling (i.e. facial masculinity) and fertilit…
Use of the Sperm-Class Analyser® for objective assessment of human sperm morphology
The Sperm-Class Analyser was validated for assessing morphometric parameters of the head and midpiece of unwashed and washed human ejaculated spermatozoa from volunteers providing a wide range of semen quality. A higher proportion of sperm could be assessed (86% fresh semen and 75% washed sperm) if Hemacolor staining was used rather than DiffQuik (80 and 73%) or Papanicolaou (78 and 68%). Different stains employed different fixatives and the area, length, width and perimeter of the sperm head was significantly larger for washed sperm stained by Hemacolor and DiffQuik. Acrosomal area ranged from 48 to 51% of the sperm head area and this percentage was larger for washed sperm stained with Dif…
Descriptive Dermoscopic Study of Depigmentation in Melanocytic Nevi Without a Visible Halo
Background and objectives: There are few cases described in the literature in which depigmentation of melanocytic nevi occurs without the appearance of halos. The aim of this study was to analyze the correlation between clinical and dermoscopic findings and to assess the usefulness of dermoscopy to identify possible markers of complete regression in melanocytic lesions. Materials and methods: A prospective, observational, descriptive study of 77 melanocytic lesions in 52 patients was undertaken over a 5-year period. Melanocytic lesions from patients who underwent periodic follow-up in the digital dermoscopy unit were analyzed if they had exhibited partial or total, permanent depigmentation …
Estudio descriptivo dermatoscópico de los nevos melanocíticos con despigmentación sin halo clínico
Resumen Introduccion la despigmentacion de nevos melanociticos sin halo perilesional se ha descrito de forma excepcional en la literatura. Material y metodos estudio observacional, descriptivo y prospectivo de 77 lesiones melanociticas correspondientes a 52 pacientes, llevado a cabo durante un periodo de 5 anos. Se han analizado las lesiones melanociticas de pacientes revisados periodicamente en la unidad de dermatoscopia digital que habian sufrido una despigmentacion permanente, ya fuera total o parcial, pero sin presentar clinicamente un halo blanquecino alrededor de los mismos. El objetivo fue estudiar la correlacion entre los hallazgos clinicos y dermatoscopicos a estudio, y establecer …
Development of mouse testis and epididymis following intrauterine exposure to a static magnetic field
In order to test if the in utero exposure to static magnetic fields affects testis and epididymis development in mice, females were exposed to 0.5–0.7 T, generated by a permanent magnet, from day 7 of gestation to the day of birth. No significant differences were found between exposed and sham-exposed animals with respect to body weight gain of dam during the gestational period, litter size, body weight of male pups at the day of birth, and body or testis-epididymis weight gain of pups from birth to day 35. Histopathologic evaluation of testis and epididymis of pups of 1, 5, 15, and 35 days of age showed no detectable alterations due to in utero exposure to static magnetic fields. Bioelectr…