0000000000362045

AUTHOR

Sybille Tachon

showing 2 related works from this author

Screening of lactic acid bacteria for reducing power using a tetrazolium salt reduction method on milk agar.

2013

WOS:000315703100020 ; www.elsevier.com/locate/jbiosc; International audience; Reducing activity is a physiological property of lactic acid bacteria (LAB) of technological importance. We developed a solid medium with tetrazolium dyes enabling weakly and strongly reducing LAB to be discriminated. It was used to quantify populations in a mixed culture (spreading method) and screen strains (spot method).

MESH: Oxidation-Reduction[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionTetrazolium Saltstetrazolium saltApplied Microbiology and Biotechnologychemistry.chemical_compoundAgarMESH: AnimalsFood science0303 health sciencesbiologyplate media04 agricultural and veterinary sciencesMESH: Tetrazolium SaltsSolid mediumLactic acidMilkMESH: AgarBiochemistryLactobacillaceaeMESH : AgarFormazanOxidation-ReductionBiotechnologyfood.ingredientMESH: LactobacillaceaeSpot methodBioengineering03 medical and health sciencesfoodoxidoreduction potentialMixed culturereducing powerAnimalsLactic AcidMESH : Tetrazolium SaltsMESH : Oxidation-Reduction030306 microbiologyscreeningMESH : Lactobacillaceae0402 animal and dairy scienceOxidation reductionbiology.organism_classification040201 dairy & animal scienceCulture MediaMESH: Milklactic acid bacteriaAgarchemistryMESH : MilkMESH : Lactic AcidMESH: Culture MediaMESH: Lactic AcidMESH : Culture MediaMESH : Animals[SDV.AEN]Life Sciences [q-bio]/Food and NutritionBacteria
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Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis

2009

The reduction of tetrazolium salts to coloured formazans is often used as an indicator of cell metabolism during microbiology studies, although the reduction mechanisms have never clearly been established in bacteria. The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate test with growing cells, and a liquid test with non-growing (resting) cells. The results showed that in both tests, TV reduction resulted from electron transfer from an intracellular donor (mainly NADH) to TV via the electron transport chain (ETC), …

DNA Bacterial[SDV]Life Sciences [q-bio]Tetrazolium SaltsMicrobiologyElectron Transport03 medical and health scienceschemistry.chemical_compoundElectron transfer030304 developmental biology0303 health sciencesbiology030306 microbiologyLactococcus lactisNADH dehydrogenaseNADH DehydrogenaseVitamin K 2biology.organism_classificationNADElectron transport chainCulture MediaLactococcus lactisMutagenesis InsertionalMembranechemistryBiochemistryGenes Bacterialbiology.proteinFormazanOxidation-ReductionIntracellularBacteria
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