Endothelial nitric oxide synthase gene polymorphisms and cardiovascular damage in hypertensive subjects: an Italian case-control study

Abstract Background Nitric oxide (NO) synthesized by endothelial nitric oxide synthase (eNOS) plays an important role in regulation of endothelial function and in the control of blood pressure. However, the results from some studies on the association between three clinically relevant eNOS gene polymorphisms (G894T, T786C and intron 4b/a) and essential hypertension are unclear. We designed a case-control study to evaluate the influence of eNOS polymorphisms on target organ damage in 127 hypertensives and 67 normotensives. Clinical evaluation, biochemical parameters, Urinary Albumin Excretion (UAE) and echocardiogram were performed to characterize target organ damage. eNOS polymorphism were …

Extraction of bioactive compounds from shrimp waste for nutraceutical application

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Additional file 2: Figure S2. of Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

Western blotting of (a) p53, (b) hsp70 and (c) TNF proteins detected in normal fibroblast HS-68 cell lines after 48 h exposure to a 0.1% concentration of Anisakis excretory/secretory (ES) and crude extract (EC) products: Lane 1: control; Lane 2: ES exposure; Lane 3: EC exposure, Std, mix of standard proteins as molecular markers. *P 

Additional file 2: Figure S2. of Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

Western blotting of (a) p53, (b) hsp70 and (c) TNF proteins detected in normal fibroblast HS-68 cell lines after 48 h exposure to a 0.1% concentration of Anisakis excretory/secretory (ES) and crude extract (EC) products: Lane 1: control; Lane 2: ES exposure; Lane 3: EC exposure, Std, mix of standard proteins as molecular markers. *P 

Estensione della definizione di Malattia di Fabry in base ai dati ottenuti dallo studio dell'attività enzimatica e degli aplotipi genetici

BIOMARKERS OF OXIDATIVE STRESS AS AN EARLY WARNING SYSTEM TO DETECT IN VITRO XENOBIOTICS’ TOXICITY: EFFECTS OF SUB LETHAL DOSES OF BIS (2-CHLORO-1-METHYLETHYL) ETHER IN CELL CULTURE

Fish oils influence adipogenesis in preadipocytes of European sea bass (Dicentrarchus Labrax), in relation to the incidence of omega-3 fatty acids

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Novel alpha-galactosidase A mutation in a female with recurrent strokes.

Abstract Anderson–Fabry disease (AFD) is an X-linked inborn error of glycosphingolipid catabolism resulting from the deficient activity of the lysosomal exoglycohydrolase, a-galactosidase A. The complete genomic and cDNA sequences of the human alpha-galactosidase A gene have been determined and to date, several disease-causing alpha-galactosidase A mutations have been identified, including missense mutations, small deletions/insertions, splice mutations, and large gene rearrangements We report a case of a 56-year-old woman with recurrent cryptogenic strokes. Ophthalmological examination revealed whorled opacities of the cornea (cornea verticillata) and dilated tortuous conjunctival vessels.…

Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

Background In countries with elevated prevalence of zoonotic anisakiasis and high awareness of this parasitosis, a considerable number of cases that associate Anisakis sp. (Nematoda, Anisakidae) and different bowel carcinomas have been described. Although neoplasia and embedded larvae were observed sharing the common site affected by chronic inflammation, no association between the nematode and malignancy were directly proved. Similarly, no data are available about the effect of secretory and excretory products of infecting larvae at the host’s cellular level, except in respect to allergenic interaction. Methods To test the mechanisms by which human non-immune cells respond to the larvae, w…

De novo mutation in a male patient with Fabry disease: a case report

Abstract Background Fabry disease is an X-linked inherited metabolic condition where the deficit of the α-galactosidase A enzyme, encoded by the GLA gene, leads to glycosphingolipid storage, mainly globotriaosylceramide. To date, more than 600 mutations have been identified in human GLA gene that are responsible for FD, including missense and nonsense mutations, small and large deletions. Such mutations are usually inherited, and cases of de novo onset occur rarely. Case presentation In this article we report an interesting case of a 44-year-old male patient suffering from a severe form of Fabry disease, with negative family history. The patient showed signs such as cornea verticillata, ang…

Parietaria judaica pollen: hypoallergenic fragment of Parj2 used as a possible tool for a vaccine strategy

Hydrogen peroxide in endothelium: multifaceted roles in cellular stress and signalling.

Additional file 1: Figure S1. of Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

Regression analysis between cells vitality and ROS production at 48 h in control cells (CO) and after treatment with excretory/secretory (ES) and crude extract (EC) products (n = 9 for each treatment). (TIF 179 kb)