0000000000365518

AUTHOR

Vincent A. Fischetti

showing 2 related works from this author

Importance of Propionibacterium acnes hemolytic activity in human intervertebral discs: A microbiological study

2018

Most patients with chronic lower back pain (CLBP) exhibit degenerative disc disease. Disc specimens obtained during initial therapeutic discectomies are often infected/colonized with Propionibacterium acnes, a Gram-positive commensal of the human skin. Although pain associated with infection is typically ascribed to the body's inflammatory response, the Gram-positive bacterium Staphylococcus aureus was recently observed to directly activate nociceptors by secreting pore-forming α-hemolysins that disrupt neuronal cell membranes. The hemolytic activity of P. acnes in cultured disc specimens obtained during routine therapeutic discectomies was assessed through incubation on sheep-blood agar. T…

Bacterial DiseasesSensory ReceptorsPhysiologyStaphylococcusCellSocial SciencesHuman skinmedicine.disease_causePathology and Laboratory MedicineToxicologyMass SpectrometryAnalytical ChemistryPathogenesis0302 clinical medicineSpectrum Analysis TechniquesINFECTIONMedicine and Health SciencesNERVEAgarToxinsPsychologyStaphylococcus AureusIntervertebral DiscPOPULATIONMammals030222 orthopedicsMultidisciplinarybiologyQSTAPHYLOCOCCUSREukaryotaNociceptorsASSOCIATIONMatrix-Assisted Laser Desorption Ionization Mass SpectrometryRuminantsPREVALENCE3. Good healthBody FluidsBacterial PathogensChemistrymedicine.anatomical_structureBloodInfectious DiseasesStaphylococcus aureusMedical MicrobiologyPhysical SciencesVertebratesMedicineSensory PerceptionAnatomyPathogensLOW-BACK-PAINResearch ArticleSignal Transductionfood.ingredientScienceLower Back PainToxic AgentsPainResearch and Analysis MethodsMicrobiologyHemolysisDegenerative disc diseaseMicrobiology03 medical and health sciencesPropionibacterium acnesfoodSigns and SymptomsDiagnostic MedicinemedicineAnimalsHumansPropionibacterium acnesMicrobial PathogensStaphylococcal InfectionGram-Positive Bacterial InfectionsINNERVATIONSheepBacteriabusiness.industryOrganismsBiology and Life SciencesCell Biologymedicine.diseasebiology.organism_classificationAmniotesChronic DiseasebusinessLow Back Pain030217 neurology & neurosurgeryBacteriaNeurosciencePLoS ONE
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Identification of a Ligand on the Wip1 Bacteriophage Highly Specific for a Receptor on Bacillus anthracis

2013

ABSTRACT Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highly specific to only a few hosts within the same bacterial species. The cellular determinants that account for the observed specificity remain unknown. Here we present the genome sequence of Wip1, a tectivirus that infects the pathogen Bacillus anthracis . The Wip1 genome is related to other tectiviruses with Gram-positive hosts, notably, AP50, but displays some interest…

Gene Expression Regulation ViralMolecular Sequence DataGenome ViralBiologyLigandsMicrobiologyGenomeBacteriophageSpecies SpecificityCloning MolecularMolecular BiologyGenomic organizationGeneticsTectivirusGene Expression Regulation BacterialArticlesLigand (biochemistry)biology.organism_classificationBacillus anthracisMicroscopy FluorescenceCapsidBacillus anthracisDNA ViralReceptors VirusTectiviridaeTectiviridaeJournal of Bacteriology
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