0000000000365758
AUTHOR
Antonio Emidio Fortunato
Supplementary Fig. 4 A Respiration and photosynthesis in P. tricornutum cells from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.
Direct assessment of oxygen consumption by a polarographic approach in both phototrophy (black bar) and mix-otrophy (red bar). B. Fluorescent based-assay to monitoring the changes in respiration using the Redox Dye A in presence of the selected compounds (see methods).
Supplementary Fig. 1 Quantitative analysis of P. tricornutum glycerolipids from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.
TAG profile in a total lipid extract from cells grown in replete conditions (A) and deplete conditions (B) in both mixotrophic and phototrophic mode. Glycerolipids are expressed in nmol / mg of dry cells. Each result is the average of two biological replicates ± SD. PHOT: light in N-replete condition; PHOTO-N: light in N-deplete condi-tion; MIX: light+glycerol in N-replete condition; MIX-N: light+glycerol in N-deplete condition.
Supplementary Fig. 2 Membrane lipid composition in P. tricornutum from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.
Lipid analysis of cells grow in N-replete conditions and N-deplete conditions in both mixotrophic and phototrophic mode. Each result is the average of two biological replicates ± SD. SQDG, sulfoquinovosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; MGDG, monogalactosyldiacylglycerol; PC, phosphatidylcholine; PHOT: light in N-replete condition; PHOTO-N: light in N-deplete condition; MIX: light+glycerol in N-replete condition; MIX-N: light+glycerol in N-deplete condition.
Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.
Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum , using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affect…
Supplementary Fig. 3 Quantification of intracellular pyruvate by a fluorescence-based method from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum
A. Pyruvate standard curve. B. Quantification of intracellular pyruvate in cells grown in phototrophy (PHOT) and mixotrophy (MIX).
A peek inside the neurosecretory brain throughOrthopedialenses
The wealth of expression and functional data presented in this overview discloses the homeogene Orthopedia (Otp) as critical for the development of the hypothalamic neuroendocrine system of vertebrates. Specifically, the results depict the up-to-date portrait of the regulation and functions of Otp. The development of neuroendocrine nuclei relies on Otp from fish to mammals, as demonstrated for several peptide and hormone releasing neurons. Additionally, the activity of Otp is essential for the induction of the dopaminergic phenotype in the hypothalamus of vertebrates. Recent insights into the pathways required for Otp regulation have revealed the implication of the main extracellular signal…
09-P102 Somitogenesis and development of primary motor neurons: The role of the homeobox uncx4.1
The gene uncx4.1 is a paired-type homeobox transcription factor, expressed during zebrafish embryonic development in branchial arches, somites, CNS and pronephric ducts. In particular, the expression at the somite level is detectable from 5ss stage and becomes progressively posteriorly and ventrally restricted to the presumptive myoblast cells in later stages. Interestingly, the progressive restriction of uncx4.1 activity anticipates and accompanies the appearance of the first outgrowing primary motor axons. Indeed, after uncx4.1 expression is not detected anymore in medial somitic cells, a ventrally projecting CaP motor axons emerge from the ventro-lateral spinal cord. In relation with mus…
Supplementary Fig. 5 Screening of mixotrophic efficiency by biolog and redox dye assay in P. tricornutum from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.
A. OD750 nm changes (relative to phototrophic growth) of P. tricornutum cells grown for 6 days in BiologTM plates P1 and PM2A that contains 190 carbon compounds (see methods). Each data point represents a different com-pound. B. Growth profile of P. tricornutum on few selected compounds (at 20 mM) and a phototrophic control in 100 mL flasks. C. Areas under the growth curves of Supplementary Fig. 5B normalized to the area of the curve of phototrophic growth.