0000000000368914

AUTHOR

Marina Blazquez

showing 3 related works from this author

Silencing of hepatic fate-conversion factors induce tumorigenesis in reprogrammed hepatic progenitor-like cells

2016

Abstract Background Several studies have reported the direct conversion of mouse fibroblasts to hepatocyte-like cells with different degrees of maturation by expression of hepatic fate-conversion factors. Methods We have used a combination of lentiviral vectors expressing hepatic fate-conversion factors with Oct4, Sox2, Klf4, and Myc to convert mouse embryonic fibroblasts into hepatic cells. Results We have generated hepatic cells with progenitor-like features (iHepL cells). iHepL cells displayed basic hepatocyte functions but failed to perform functions characteristic of mature hepatocytes such as significant Cyp450 or urea cycle activities. iHepL cells expressed multiple hepatic-specific …

0301 basic medicineMaleCarcinogenesisCellular differentiationMedicine (miscellaneous)Gene ExpressionReceptors G-Protein-CoupledMiceMice Inbred NODHepatocyteTransgenesStem CellsTeratomaCell DifferentiationForkhead Transcription FactorsCellular ReprogrammingCell biologyKLF4Molecular MedicineStem cellReprogrammingDirect reprogrammingGenetic VectorsKruppel-Like Transcription FactorsBiologyBiochemistry Genetics and Molecular Biology (miscellaneous)Proto-Oncogene Proteins c-myc03 medical and health sciencesKruppel-Like Factor 4SOX2AnimalsHepatectomyGene SilencingProgenitor cellResearchXenograftSOXB1 Transcription FactorsLentivirusCD24 AntigenCell BiologyFibroblastsEmbryo MammalianEmbryonic stem cell030104 developmental biologyTumorigenesisHepatic stellate cellHepatocytesOctamer Transcription Factor-3BiomarkersProgenitorStem Cell Research & Therapy
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Gata4 Blocks Somatic Cell Reprogramming By Directly Repressing Nanog

2012

Abstract Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by ectopic expression of the four factors Oct4, Klf4, Sox2, and Myc. Here, we investigated the role of Gata4 in the reprogramming process and present evidence for a negative role of this family of transcription factors in the induction of pluripotency. Coexpression of Gata4 with Oct4, Klf4, and Sox2 with or without Myc in mouse embryonic fibroblasts greatly impaired reprogramming and endogenous Nanog expression. The lack of Nanog upregulation was associated with a blockade in the transition from the initiation phase of reprogramming to the full pluripotent state characteristic of iPS cells. Addition of Nanog …

Pluripotent Stem CellsTranscriptional ActivationHomeobox protein NANOGChromatin ImmunoprecipitationTranscription GeneticRex1Kruppel-Like Transcription FactorsDown-RegulationElectrophoretic Mobility Shift AssayBiologyCell LineProto-Oncogene Proteins c-mycKruppel-Like Factor 4MiceSOX2AnimalsRNA MessengerRNA Small InterferingInduced pluripotent stem cellEmbryonic Stem Cellsreproductive and urinary physiologyHomeodomain ProteinsSOXB1 Transcription FactorsNanog Homeobox ProteinCell DifferentiationNanog Homeobox ProteinCell BiologyCellular ReprogrammingEmbryonic stem cellGATA4 Transcription FactorKLF4embryonic structuresHepatocyte Nuclear Factor 3-betaCancer researchMolecular MedicineRNA Interferencebiological phenomena cell phenomena and immunityOctamer Transcription Factor-3ReprogrammingDevelopmental BiologyStem Cells
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Additional file 1: of Silencing of hepatic fate-conversion factors induce tumorigenesis in reprogrammed hepatic progenitor-like cells

2016

Supplementary information. (PDF 923 kb)

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