Endometrial Receptivity Analysis (ERA): data versus opinions

Abstract This article summarises and contextualises the accumulated basic and clinical data on the ERA test and addresses specific comments and opinions presented by the opponent as part of an invited debate. Progress in medicine depends on new technologies and concepts that translate to practice to solve long-standing problems. In a key example, combining RNA sequencing data (transcriptomics) with artificial intelligence (AI) led to a clinical revolution in personalising disease diagnosis and fostered the concept of precision medicine. The reproductive field is no exception. Translation of endometrial transcriptomics to the clinic yielded an objective definition of the limited time period …

INCREASED CONCORDANCE RATES BETWEEN EMBRYO CELL-FREE DNA AND TROPHECTODERM BIOPSIES ARE RELATED TO FEMALE AGE INDEPENDENTLY OF CULTURE CONDITIONS

O-126 Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Abstract Study question Is there an association between the composition of the endometrial microbiota and the reproductive outcomes in infertile patients undergoing in vitro fertilization (IVF)? Summary answer The composition of the endometrial microbiota (EM) prior to embryo transfer is associated with the different reproductive outcomes: live birth, no pregnancy or clinical miscarriage. What is known already The investigation of bacterial communities in the female reproductive tract using molecular methods has revealed the existence of a continuum microbiota that extends from the vagina to the upper genital tract. Previous evidence suggests the existence of an association between the vagi…

Additional file 7 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 6 : Supplementary Table 3. Sequencing reads obtained from endometrial fluid and endometrial biopsy samples.

Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endomet…

Window of implantation transcriptomic stratification reveals different endometrial subsignatures associated with live birth and biochemical pregnancy

Objective To refine the endometrial window of implantation (WOI) transcriptomic signature by defining new subsignatures associated to live birth and biochemical pregnancy. Design Retrospective cohort study. Setting University-affiliated in vitro fertilization clinic and reproductive genetics laboratory. Patient(s) Healthy fertile oocyte donors (n = 79) and patients with infertility diagnosed by Endometrial Receptivity Analysis (n = 771). Intervention(s) None. Main Outcome Measure(s) WOI transcriptomic signatures associated with specific reproductive outcomes. Result(s) The retrospective cohort study was designed to perform a prediction model based on transcriptomic clusters for endometrial …

Additional file 8 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 7 : Supplementary Table 4. Bacteria detected in endometrial fluid and endometrial biopsy samples after filtering for low abundance taxa and potential contaminants.

Derivation of clinical-grade human embryonic stem cells.

Embryonic stem cells proliferate in vitro while maintaining an undifferentiated state, and are capable of differentiating into most cell types under appropriate conditions. These properties imply great potential in the treatment of various diseases and disabilities. In fact, the first clinical trials with hESC for treating spinal cord injuries will begin next year. However, therapeutic application of human embryonic stem cell derivatives is compromised by the exposure of existing lines to animal and human components, with the subsequent risk of contamination with retroviruses and other pathogens, which can be transmitted to patients. The scientific community is striving to avoid the use of …

A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF.

Abstract Research question Does clinical performance of personalized embryo transfer (PET) guided by endometrial receptivity analysis (ERA) differ from frozen embryo transfer (FET) or fresh embryo transfer in infertile patients undergoing IVF? Design Multicentre, open-label randomized controlled trial; 458 patients aged 37 years or younger undergoing IVF with blastocyst transfer at first appointment were randomized to PET guided by ERA, FET or fresh embryo transfer in 16 reproductive clinics. Results Clinical outcomes by intention-to-treat analysis were comparable, but cumulative pregnancy rate was significantly higher in the PET (93.6%) compared with FET (79.7%) (P = 0.0005) and fresh embr…

Defining the genomic signature of totipotency and pluripotency during early human development.

The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation netw…

Additional file 5 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 4 : Supplementary Table 1. Sociodemographic and clinical characteristics of study participants.

In vitro fertilization with personalized blastocyst transfer versus frozen or fresh blastocyst transfer: a multicenter, randomized clinical trial

Additional file 6 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 5 : Supplementary Table 2. Clinical variables in patients with different reproductive outcomes.

Additional file 4 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 3 : Supplementary Figure 3. Confidence intervals and reference ranges of bacterial taxa detected in patients with live birth. The endometrial microbiome from patients with a live birth was analysed in (A) endometrial fluid and (B) endometrial biopsy samples to determine the reference ranges for each evaluated taxon. The confidence interval displays the relative abundance for all assessed patients, revealing the healthy ranges of abundance for the taxa in the tested panel. The healthy distribution was used to define the 95% confidence interval (red line) and taxa abundance in patients with poor reproductive outcomes���no pregnancy, biochemical pregnancy, and clinical miscarri…

Additional file 3 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 2 : Supplementary Figure 2. Taxa enriched in patients with different reproductive outcomes.

Additional file 2 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Additional file 1 : Supplementary Figure 1. Taxa found in endometrial fluid (A) and endometrial biopsy (B) samples.