0000000000373978

AUTHOR

Ana M Oltra

showing 3 related works from this author

Antioxidant enzyme activities and the production of MDA and 8-oxo-dG in chronic lymphocytic leukemia.

2001

Abstract Chronic lymphocytic leukemia (CLL) is a neoplastic disease susceptible to antioxidant enzyme alterations and oxidative stress. We have examined the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and the oxidized/reduced glutathione (GSSG/GSH) ratio together with the levels of malondialdehyde (MDA) and 8-oxo-2′-deoxyguanosine (8-oxo-dG) in lymphocytes of CLL patients and compared them with those of normal subjects of the same age. SOD and CAT activity decreased in CLL lymphocytes while GPx activity increased. GSH content of CLL lymphocytes also increased, and GSSG concentration remained constant. Thus, a reduced GSSG/GSH ratio was obtaine…

Malemedicine.medical_specialtyAntioxidantmedicine.medical_treatmentChronic lymphocytic leukemiamedicine.disease_causeBiochemistryAntioxidantsSuperoxide dismutasechemistry.chemical_compoundhemic and lymphatic diseasesPhysiology (medical)Internal medicineMalondialdehydemedicineHumansLymphocytesAgedchemistry.chemical_classificationGlutathione PeroxidasebiologySuperoxide DismutaseGlutathione peroxidaseDeoxyguanosineGlutathioneDNA NeoplasmMiddle AgedMalondialdehydemedicine.diseaseCatalaseGlutathioneLeukemia Lymphocytic Chronic B-CellEndocrinologychemistryBiochemistryCatalase8-Hydroxy-2'-Deoxyguanosinebiology.proteinFemaleLipid PeroxidationOxidoreductasesOxidative stressDNA DamageFree radical biologymedicine
researchProduct

Oxidative stress induces the expression of the major histocompatibility complex in murine tumor cells.

2001

The effect of t-butyl hydroperoxide (t-BOOH) on the induction of the Major Histocompatibility Complex (MHC) class I genes has been studied in two cell clones (B9 and G2) of the methylcholanthrene-induced murine fibrosarcoma GR9. These two clones were selected based on their different biological and biochemical behavior specially related to their tumor induction capability when injected into a BALB/c mouse. t-BOOH (0.125 mM) induced the expression of H-2 molecules in both cell clones. In B9 cell clone, in which MHC basal expression is very low or absent, t-BOOH significantly induced H-2Kd, H-2Dd and H-2Ld molecules. In G2 cell clone the expression of MHC class I genes was also enhanced by th…

FibrosarcomaCellElectrophoretic Mobility Shift AssayBiologyMajor histocompatibility complexBiochemistryMajor Histocompatibility ComplexTransactivationMiceAntigentert-ButylhydroperoxideCell CloneMalondialdehydeMHC class ImedicineTumor Cells CulturedAnimalsGlutathione PeroxidaseMice Inbred BALB CSuperoxide DismutaseMHC Class I GeneHistocompatibility Antigens Class INF-kappa BDeoxyguanosineGeneral Medicine3T3 CellsCatalaseFlow CytometryMolecular biologyGlutathioneOxidative Stressmedicine.anatomical_structureGene Expression Regulation8-Hydroxy-2'-Deoxyguanosinebiology.proteinCD8MethylcholanthreneFree radical research
researchProduct

Role of glutathione in the induction of apoptosis and c-fos and c-jun mRNAs by oxidative stress in tumor cells.

2003

We have used two tumor cell clones (B9 and G2), derived from the methylcholanthrene-induced murine fibrosarcoma GR9 and normal BALB/c3T3 fibroblasts, to study the ability of t-BOOH derived reactive oxygen radicals to induce oxidative stress, apoptosis and c-fos and c-jun mRNA transcription. These clones differ in terms of their major histocompatibility complex (MHC) (H-2) class I genes expression, their tumor induction and metastatic potential and their reduced glutathione (GSH) levels. Incubation of both cell clones in the presence of t-BOOH results in the increase of 8-oxo-2'-deoxyguanosine (8-oxo-dG) and malondialdehyde and the decrease of GSH. The xenobiotic also induces the transcripti…

Cancer ResearchBALB 3T3 CellsTranscription GeneticProto-Oncogene Proteins c-junFibrosarcomaCellApoptosisBiologymedicine.disease_causeAntioxidantsSuperoxide dismutasechemistry.chemical_compoundMicetert-ButylhydroperoxideCell CloneMalondialdehydemedicineTumor Cells CulturedAnimalsRNA MessengerDNA Primerschemistry.chemical_classificationReactive oxygen speciesReverse Transcriptase Polymerase Chain Reactionc-junHistocompatibility Antigens Class IDeoxyguanosineGlutathioneFibroblastsMolecular biologyGlutathioneOxidative Stressmedicine.anatomical_structureOncologychemistryGene Expression RegulationApoptosis8-Hydroxy-2'-Deoxyguanosinebiology.proteinReactive Oxygen SpeciesProto-Oncogene Proteins c-fosOxidative stressCancer letters
researchProduct