0000000000377315
AUTHOR
Miguel Bolonio
MOESM2 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 2: Table S1. Primers used for qRT-PCR.
MOESM1 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 1: Figure S1. Cell sorting strategy using BDFACSAriaIIIâ ˘ cell sorter. Figure S2. Validation of the polycistroniclentiviral vector in HDF.
Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Abstract Background Human fibroblasts can be reprogrammed into induced hepatocyte-like cells through the expression of a set of transcription factors. Although the generation of induced hepatocyte-like cells by HNF4A, HNF1A, and FOXA3 expression has proven to be a robust experimental strategy, using multiple lentivirus results in a highly variable heterogeneous population. Methods We designed and implemented a novel approach based on the delivery of reprogramming factors and green fluorescent protein in a single doxycycline-inducible lentiviral vector using 2A self-cleaving peptides. Results Fibroblasts infected with the lentiviral vector can be amplified in basic fibroblast culture media i…
Improved in vivo efficacy of clinical-grade cryopreserved human hepatocytes in mice with acute liver failure.
Clinical hepatocyte transplantation short-term efficacy has been demonstrated; however, some major limitations, mainly due to the shortage of organs, the lack of quality of isolated cells and the low cell engraftment after transplantation, should be solved for increasing its efficacy in clinical applications. Cellular stress during isolation causes an unpredictable loss of attachment ability of the cells, which can be aggravated by cryopreservation and thawing. In this work, we focused on the use of a Good Manufacturing Practice (GMP) solution compared with the standard cryopreservation medium, the University of Wisconsin medium, for the purpose of improving the functional quality of cells …
MOESM1 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 1: Figure S1. Cell sorting strategy using BDFACSAriaIIIâ ˘ cell sorter. Figure S2. Validation of the polycistroniclentiviral vector in HDF.