0000000000379528

AUTHOR

José Pontón

showing 4 related works from this author

Candida biotypes in patients with oral leukoplakia and lichen planus. Candida biotypes in leukoplakia and lichen planus.

1996

Prevalence of yeasts in 35 leukoplakia and 34 oral lichen planus patients was compared with that observed in persons without oral diseases. Serotype and morphotype were determined on Candida albicans isolates. Yeasts were isolated from the oral cavity specimens of 43.7% of the patients. C. albicans (serotype A) was the predominant species (76% in leukoplakia, 88.2% in lichen planus and 60.8% in healthy persons). Sixteen morphotypes were encountered on malt extract agar, being 732, 733, 734, 753 and 754 the most frequently found. Morphotypes SP1N and SP1Y were the most common on Sabouraud-trypheniltetrazolium agar (68.4% of the isolates from leukoplakia and 73.3% from lichen planus, but only…

SerotypeAdultMaleVeterinary (miscellaneous)BiologyApplied Microbiology and BiotechnologyMicrobiologyMicrobiologystomatognathic systemCandida albicansmedicineHumansOral mucosaskin and connective tissue diseasesCandida albicansMycological Typing TechniquesLeukoplakiaCandidaMouthintegumentary systemFungi imperfectiMiddle Agedmedicine.diseasebiology.organism_classificationCorpus albicansCulture Mediastomatognathic diseasesmedicine.anatomical_structureOral microbiologyOral lichen planusFemaleLeukoplakia OralAgronomy and Crop ScienceLichen Planus OralMycopathologia
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Antibody Complementarity-Determining Regions (CDRs) Can Display Differential Antimicrobial, Antiviral and Antitumor Activities

2008

9 p. Background: Complementarity-determining regions (CDRs) are immunoglobulin (Ig) hypervariable domains that determine specific antibody (Ab) binding. We have shown that synthetic CDR-related peptides and many decapeptides spanning the variable region of a recombinant yeast killer toxin-like antiidiotypic Ab are candidacidal in vitro. An alanine-substituted decapeptide from the variable region of this Ab displayed increased cytotoxicity in vitro and/or therapeutic effects in vivo against various bacteria, fungi, protozoa and viruses. The possibility that isolated CDRs, represented by short synthetic peptides, may display antimicrobial, antiviral and antitumor activities irrespective of Ab…

Antifungal AgentsBIOCHEMISTRY AND MOLECULAR BIOLOGYMolecular Sequence DataImmunologylcsh:MedicineAntineoplastic AgentsMicrobial Sensitivity TestsComplementarity determining regionBiologyAntiviral AgentsOncology/Skin CancersAntibodiesMiceMicrobiology/Applied MicrobiologyAntigenBiochemistry/Protein ChemistryInfectious Diseases/Fungal InfectionsIn vivoCell Line TumorCandida albicansInfectious Diseases/Viral InfectionsAnimalsHumansAmino Acid Sequencelcsh:SciencePeptide sequenceMultidisciplinaryMEDICINElcsh:RAntimicrobialComplementarity Determining RegionsVirologyIn vitroOncologyBiochemistryViral replicationAGRICULTURAL AND BIOLOGICAL SCIENCESVirology/Immunodeficiency VirusesHIV-1biology.proteinlcsh:QAntibodyResearch ArticlePLoS ONE
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Comparison of morphotypic and genotypic methods for strain delineation inCandida

1998

Summary. We compared two phenotypic methods, colony morphotyping on Sabouraud-tripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans strains from culture collections. Analysis of isolates on STTZ showed 11 different morphotypes. In two patients there was a switch in the morphotype coincidential with a change in the susceptibility of the isolates to azole antifungals. C. albicans isolates were divided into two serotypes. Sixteen and 18 different patterns were identified among the Candida isolates by k…

GenotypebiologyStrain (chemistry)CandidiasisMicrobial Sensitivity TestsDermatologyGeneral MedicineFungi imperfectibiology.organism_classificationCorpus albicansRandom Amplified Polymorphic DNA TechniqueMicrobiologylaw.inventionPhenotypeInfectious DiseaseslawKaryotypingGenotypeTypingSerotypingMycological Typing TechniquesCandida albicansGenotypingPolymerase chain reactionCandidaMycoses
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Isolation and characterization of anavirulent Candida albicansyeast monomorphic mutant

2003

Mutagenesis of Candida albicans strain ATCC 26555 with N-methyl-nitro-N-nitrosoguanidine followed by plating on solid yeast nitrogen base-N-acetylglucosamine medium at 37 degrees C yielded colony morphology variants that were characterized as forming smooth colonies, in contrast to the rough colonies formed by the parental strain. One yeast monomorphic mutant, CAL4, was studied in detail. Strain CAL4 is defective in filamentous growth, unable to form hyphae or pseudohyphae in vivo and in vitro. These filamentous structures are not elicited by commonly used external stimuli such as serum. The mutant had no obvious alterations in its mannan, glucan or chitin content. The total quantity of non…

ElectrophoresisMaleVirulenceHyphaStrain (chemistry)biologyMutantCandidiasisMutagenesis (molecular biology technique)General Medicinebiology.organism_classificationYeastMicrobiologyFungal ProteinsCell wallMiceInfectious DiseasesCell WallCandida albicansMutationAnimalsCandida albicansMannanMedical Mycology
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