OR 43 Influence of root canal sealers on inflammatory reactions in cell cultures

Dimensional variability of nonstandardized greater taper finger spreaders with matching gutta-percha points

Aim The purpose of this research was to determine the compatibility and dimensional variability between nonstandardized gutta-percha points and matching finger spreaders. Methodology The diameters of nonstandardized gutta-percha points (n = 15) and matching finger spreaders (n = 15) from different manufacturers (Kerr, Dentsply Maillefer, Vereignigte Dentalwerke and Roeko) were determined and statistically analysed using a profile projector under a magnification of 50 × (±0.002 mm). Results The dimensions of the finger spreaders and gutta-percha cones were inconsistent. Of the 29 groups of nonstandardized gutta-percha cones evaluated, 22 had standard deviations larger than 0.020 at D1. The s…

Effect of apical root-end filling materials on gingival fibroblasts

AIM To determine the influence of root-end filling materials on specific cellular responses of gingival fibroblasts (GF). METHODOLOGY The reactions of cells in contact with mineral trioxide aggregate (MTA), amalgam and a chemically inert titanium alloy were determined based on the assessment of prostaglandin (PGE2) release with and without arachidonic acid stimulation, protein and lactate synthesis, and cell proliferation. Cells cultured without test materials served as controls (100%). RESULTS The fibroblasts showed a highly significant decrease in protein synthesis when in contact with amalgam (61.8 +/- 13.6%); MTA (91.2 +/- 5.9%) and titanium (92.4 +/- 4.7%) had little effect on this par…

OR 62 Are electronic apex locators reliable? An in vitro study