0000000000386152

AUTHOR

Ronald Osinga

showing 2 related works from this author

What to do in marine biotechnology?

2003

Este artículo contiene 5 páginas, 1 figura

Bio Process Engineeringbusiness.industryBioengineeringBiologyBiotechnologyPRI BioscienceHuman healthBrainstormingMarine biotechnologyBrainstormSession (computer science)businessMolecular BiologyFutureBiotechnologyVLAGBiomolecular Engineering
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Selenium affects biosilica formation in the demosponge Suberites domuncula

2005

Selenium is a trace element found in freshwater and the marine environment. We show that it plays a major role in spicule formation in the demosponge Suberites domuncula. If added to primmorphs, an in vitro sponge cell culture system, it stimulates the formation of siliceous spicules. Using differential display of transcripts, we demonstrate that, after a 72-h exposure of primmorphs to selenium, two genes are up-regulated; one codes for selenoprotein M and the other for a novel spicule-associated protein. The deduced protein sequence of selenoprotein M (14 kDa) shows characteristic features of metazoan selenoproteins. The spicule-associated protein (26 kDa) comprises six characteristic repe…

SpiculeBlotting WesternMolecular Sequence DataFluorescent Antibody Techniquechemistry.chemical_elementselenium; silica; silicatein; spicules; spongesBiochemistryAntibodiesSeleniumSponge spiculeDemospongeAnimalsAmino Acid SequenceSelenoproteinsMolecular Biologychemistry.chemical_classificationGlutathione PeroxidaseBase SequencebiologyGene Expression ProfilingProteinsCell BiologyAnatomySilicon Dioxidebiology.organism_classificationCathepsinsUp-RegulationAmino acidSuberites domunculaSpongeBiochemistrychemistrySelenoproteinSuberitesSeleniumFEBS Journal
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