0000000000388668

AUTHOR

Uta-maria Bauer

showing 3 related works from this author

The murine nuclear orphan receptor GCNF is expressed in the XY body of primary spermatocytes

1998

AbstractWe have studied the expression of the nuclear orphan receptor GCNF (germ cell nuclear factor) on the mRNA and protein level in pubertal and adult mouse testes. We show by Northern and Western blot analyses and by in situ hybridization that GCNF is expressed in spermatocytes and round spermatids of adult mouse testis suggesting that GCNF may be a transcriptional regulator of spermatogenesis. Since the GCNF protein is accumulated in the XY body of late pachytene spermatocytes, it may be involved in transcriptional inactivation of sex chromosomes.

MaleTranscription GeneticGerm cell nuclear factorBiophysicsFluorescent Antibody TechniqueReceptors Cytoplasmic and NuclearIn situ hybridizationBiologyIn Vitro TechniquesGerm cell nuclear factorBiochemistryEpitheliumMiceWestern blotStructural BiologySpermatocytesNuclear Receptor Subfamily 6 Group A Member 1TestisGeneticsTranscriptional regulationmedicineAnimalsRNA MessengerRetinoid receptor-related testis-associated receptorSpermatogenesisMolecular BiologyOrphan receptorMessenger RNAMice Inbred BALB Cmedicine.diagnostic_testCell BiologyMolecular biologySpermatidsNeuron-derived orphan receptor 1DNA-Binding ProteinsGene Expression RegulationNuclear orphan receptorSpermatogenesisNeuronal cell nuclear factorXY bodyFEBS Letters
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Neuronal Cell Nuclear Factor. A Nuclear Receptor Possibly Involved in the Control of Neurogenesis and Neuronal Differentiation

1997

We have cloned from a cDNA library of neuronal derivatives of retinoic-acid-induced embryonic carcinoma cells a nuclear receptor that may be involved in the control of late neurogenesis and early neuronal differentiation. The receptor which is practically identical in sequence with germ cell nuclear factor, has been designated neuronal cell nuclear factor (NCNF). NCNF is exclusively expressed in the neuronal derivatives of PCC7-Mz1 cells, with the expression beginning within hours of exposure to retinoic acid. In the developing mouse brain, NCNF is expressed in the marginal zones of the neuroepithelium which are known to contain young postmitotic neurons. NCNF binds to the DRO sequence ther…

Germ cell nuclear factorRetinoic acidReceptors Cytoplasmic and NuclearTretinoinBiologyLigandsBiochemistryMicechemistry.chemical_compoundNuclear Receptor Subfamily 6 Group A Member 1Tumor Cells CulturedAnimalsCloning MolecularReceptorIn Situ HybridizationNuclear receptor co-repressor 1NeuronsNeurogenesisBrainGene Expression Regulation DevelopmentalCell DifferentiationDNABlotting NorthernMolecular biologyDNA-Binding ProteinsRepressor ProteinsNeuroepithelial cellNuclear receptor coactivator 1Blotting SouthernOligodeoxyribonucleotidesnervous systemchemistryNuclear receptorEuropean Journal of Biochemistry
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Cloning of the human NCNF gene.

1998

We have cloned from a cDNA library of human testis tissue the human homologue to the mouse nuclear orphan receptor NCNF (neuronal cell nuclear factor). The open reading frame encodes a protein of 480 amino acids, the sequence of which (EMBL accession no. X99975) is 98.3% identical to the mouse homologue. Northern blot analysis of adult human tissues revealed a broad pattern of tissue expression. Similar to NCNF expression in mouse testis, two transcript forms of the single copy gene are expressed in human tissues. The two transcript forms which differ only in their 3'UTR, result in human from differential polyadenylation, in mouse from alternative splicing. Based on the high level of sequen…

AdultMaleMolecular Sequence DataReceptors Cytoplasmic and NuclearBiologyBiochemistryMiceNuclear Receptor Subfamily 6 Group A Member 1Sequence Homology Nucleic AcidTestisAnimalsHumansNorthern blotAmino Acid SequenceCloning MolecularMolecular BiologyPeptide sequenceGeneCloningOrphan receptorRegulation of gene expressionBase SequencecDNA libraryAlternative splicingCell BiologyDNAMolecular biologyDNA-Binding ProteinsRepressor ProteinsAlternative SplicingGene Expression RegulationOrgan SpecificityJournal of receptor and signal transduction research
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