The pan-transcriptome of Vicia faba

Vicia faba (faba bean) is a nitrogen-fixing protein crop that, in contrast to soya bean, yields well in temperate climates. It is diploid but has a very large genome of ~13 Gb, and genomic resources are currently limited. To provide a high-quality reference gene set and expression atlas, we have carried out RNA-seq of multiple tissues from five faba bean accessions. For each tissue, an assembly was produced using the Trinity software package using both Illumina and PacBio reads. For each accession, the assemblies were then merged to create a more complete reference gene set. The assemblies were of high quality, with more than 95% complete BUSCOs [1] and read mapping rates of ~90%. The assem…