Author: Sebastian L Wenski

0000000000394055

AUTHOR

Sebastian L Wenski

showing 3 related works from this author

Back Cover: Promoter Activation in Δ hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing (Ange…

2019

chemistry.chemical_compoundbiologyBiochemistryChemistryMetaboliteMutantINTCover (algebra)General ChemistryProteobacteriabiology.organism_classificationCatalysisAngewandte Chemie International Edition

researchProduct

Rücktitelbild: Promoter Activation in Δ hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing (A…

2019

chemistry.chemical_compoundBiochemistryChemistryMetaboliteMutantGeneral MedicineAngewandte Chemie

researchProduct

Promoter Activation in Dhfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing

2019

Abstract Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene‐sequence‐similarity‐based bioinformatics strategies, the actual access to these NPs for structure elucidation and bioactivity testing remains difficult. Deletion of the gene encoding the RNA chaperone, Hfq, results in strains losing the production of most NPs. By exchanging the native promoter of a desired BGC against an inducible promoter in Δhfq mutants, almost exclusive production of the corresponding NP from the targeted BGC in Photorhabdus, Xenorhabdus and Pseud…

bioactivity testingnatural productsMetaboliteMutantPeptide SynthetasesXenorhabdus010402 general chemistry01 natural sciencesCatalysisBiosynthesis | Very Important Paperchemistry.chemical_compoundddc:570RNA chaperoneHumansMetabolomicsGeneResearch ArticlesBiological Productsbiology010405 organic chemistryPseudomonastechnology industry and agricultureGeneral MedicineGeneral Chemistrybiology.organism_classification0104 chemical sciencesBiosynthetic PathwayseasyPACIdchemistryBiochemistryddc:540proteobacteriaPhotorhabdussimplified productionResearch Article

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