0000000000395346

AUTHOR

Siham Hichami

showing 2 related works from this author

Protein S-nitrosylation: specificity and identification strategies in plants

2015

SPE Pôle IPM UB; International audience; The role of nitric oxide (NO) as a major regulator of plant physiological functions has become increasingly evident. To further improve our understanding of its role, within the last few years plant biologists have begun to embrace the exciting opportunity of investigating protein S-nitrosylation, a major reversible NO-dependent post-translational modification (PTM) targeting specific Cys residues and widely studied in animals. Thanks to the development of dedicated proteomic approaches, in particular the use of the biotin switch technique (BST) combined with mass spectrometry, hundreds of plant protein candidates for S-nitrosylation have been identi…

[SDV]Life Sciences [q-bio]Regulatornitric oxide;S-nitrosylation;post-translational modification;plant;signaling;biotin switcht echniqueplantComputational biologyReview ArticleBiologyBioinformaticsNitric Oxidelcsh:Chemistrybiotin switcht echniqueProtein S-nitrosylationpost-translational modifications[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyFunctional studiesGeneral ChemistryS-NitrosylationPlantsS-nitrosylationStructure and functionChemistryBiotin switchpost-translational modificationlcsh:QD1-999Plant protein[SDE]Environmental SciencesBiotin Switch TechniqueIdentification (biology)signalingFrontiers in Chemistry
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S-Nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 Prevents Its Irreversible Oxidation by Hydrogen Peroxide

2022

Tyrosine-specific protein tyrosine phosphatases (Tyr-specific PTPases) are key signaling enzymes catalyzing the removal of the phosphate group from phosphorylated tyrosine residues on target proteins. This post-translational modification notably allows the regulation of mitogen-activated protein kinase (MAPK) cascades during defense reactions. Arabidopsis thaliana protein tyrosine phosphatase 1 (AtPTP1), the only Tyr-specific PTPase present in this plant, acts as a repressor of H2O2 production and regulates the activity of MPK3/MPK6 MAPKs by direct dephosphorylation. Here, we report that recombinant histidine (His)-AtPTP1 protein activity is directly inhibited by H2O2 and nitric oxide (NO) …

Arabidopsis thaliananitric oxideoxidationH2O2Plant cultureprotein tyrosine phosphatase 1S-nitrosationSB1-1110Frontiers in Plant Science
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