0000000000400219
AUTHOR
Dagnija Loča
Functionalized silver doped hydroxyapatite scaffolds for controlled simultaneous silver ion and drug delivery
Abstract Bacterial infections are a major problem in bone tissue regeneration, thus it is essential to incorporate antibacterial properties within the bone scaffolds. Silver compounds are frequently used as antibacterial agents to prevent bacterial infections and numerous studies have shown that silver ions can be incorporated within the biocompatible and osteoconductive biomaterial hydroxyapatite (HAp) structure, but, so far, no study has thoroughly evaluated silver ion release rates in long term. Therefore, we have established a novel carrier system for local drug delivery based on functionalized silver doped hydroxyapatite with determined long term silver ion release rates. Silver ions f…
FACTORS AFFECTING CHINESE HAMSTER OVARY CELL PROLIFERATION AND VIABILITY
Advantageous cultivation procedures for the Chinese hamster ovary (CHO) cells are necessary for the productive commercial production of biopharmaceuticals. A main challenge that needs to be addressed during the process development is the differences in each cell line requirements concerning the nutrients and feed strategies in order to achieve the desired growth characteristics. Therefore, within the current research, a naïve high cell density serum free suspension adapted CHO cell line was tested with glucose and glutamine rich feeds in fed-batch Erlenmeyer shake flask cultures. Glucose consumption rate was adjusted to develop the optimal feed strategies. Obtained results indicated that hi…
VISUALIZATION APPROACHES FOR STIRRED TANK BIOREACTORS
Computational Fluid Dynamics (CFD) is the analysis of fluid behaviour employing numerical solution methods. Using CFD it is possible to analyse simple and complex fluid-gas, fluid-fluid or fluid-solid interactions. Fluid dynamics is described with laws of physics in the form of partial differential equations also known as Navier-Stokes equations. Sophisticated CFD solvers transform these laws into algebraic equations which are solved by numerical methods. In this paper Ansys CFX and Fluent analysis systems as research methods are used to visualize flow patterns in a stirred tank bioreactor. The results obtained are informative and can be used to improve the yield of biomass. CFD analysis ca…