0000000000414124

AUTHOR

Ulrich Marggraf

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Configurable low-cost plotter device for fabrication of multi-color sub-cellular scale microarrays.

2014

We report on the construction and operation of a low-cost plotter for fabrication of microarrays for multiplexed single-cell analyses. The printing head consists of polymeric pyramidal pens mounted on a rotation stage installed on an aluminium frame. This construction enables printing of microarrays onto glass substrates mounted on a tilt stage, controlled by a Lab-View operated user interface. The plotter can be assembled by typical academic workshops from components of less than 15 000 Euro. The functionality of the instrument is demonstrated by printing DNA microarrays on the area of 0.5 squared centimeters using up to three different oligonucleotides. Typical feature sizes are 5 μm diam…

FabricationMaterials scienceScale (ratio)NanotechnologyMultiplexingBiomaterialsUser-Computer InterfacePlotterHumansGeneral Materials ScienceBiochipOligonucleotide Array Sequence AnalysisEGF ReceptorsEpidermal Growth FactorOligonucleotideDNA-directed protein immobilization EGF receptors device automation multiplexed patterns polymer pen lithographyGeneral ChemistryMicrofluidic Analytical TechniquesErbB ReceptorsTissue Array AnalysisCosts and Cost AnalysisMCF-7 CellsPrintingDNA microarraySingle-Cell AnalysisBiotechnologySmall (Weinheim an der Bergstrasse, Germany)
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