BythinellaMoquin-Tandon, 1856 (Gastropoda: Rissooidea: Bythinellidae) in Romania: species richness in a glacial refugium

Mitochondrial cytochrome oxidase I (COI) and ribosomal internal transcribed spacer 1 (ITS-1) sequences were analysed in 12 Romanian Bythinella populations. Phylogenetic relationships were inferred using maximum parsimony, maximum likelihood and Bayesian techniques. For COI, the Kimura two-parameter (K2P) distances and haplotype networks were computed. Two sympatric and four allopatric groups were distinguished. The K2P distances are similar to those for congeneric rissooids, so each of the six groups represents a species. Two are identified as Bythinella molcsanyi H. Wagner, 1941, and Bythinella dacica Grossu, 1946. The other four groups cannot be ascribed to any known Bythinella. The occur…