0000000000427802

AUTHOR

Toshihiro Hashimoto

showing 3 related works from this author

ERK1/2 activation in human taste bud cells regulates fatty acid signaling and gustatory perception of fat in mice and humans

2016

Obesity is a major public health problem. An in-depth knowledge of the molecular mechanisms of oro-sensory detection of dietary lipids may help fight it. Humans and rodents can detect fatty acids via lipido-receptors, such as CD36 and GPR120. We studied the implication of the MAPK pathways, in particular, ERK1/2, in the gustatory detection of fatty acids. Linoleic acid, a dietary fatty acid, induced via CD36 the phosphorylation of MEK1/2-ERK1/2-ETS-like transcription factor-1 cascade, which requires Fyn-Src kinase and lipid rafts in human taste bud cells (TBCs). ERK1/2 cascade was activated by Ca2+ signaling via opening of the calcium-homeostasis modulator-1 (CALHM1) channel. Furthermore, f…

0301 basic medicineSmall interfering RNAMouseCD36BiochemistryMapkObesechemistry.chemical_compound0302 clinical medicinegpr120Cd36Mice Knockoutchemistry.chemical_classificationGene knockdownbiologyKinaseFatty AcidsTaste PerceptionGPR120Taste BudsLipidsProtein-tyrosine kinases3. Good healthTasteBenzamidesBiotechnologymedicine.medical_specialtyMAP Kinase Signaling SystemLinoleic acid[SDV.BC]Life Sciences [q-bio]/Cellular BiologyPreferenceFood Preferences03 medical and health sciencesCalhm1Internal medicineDietary-fatGeneticsmedicineAnimalsHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyCalcium SignalingObesityMolecular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology[ SDV.BC ] Life Sciences [q-bio]/Cellular BiologyResearchDiphenylamineFatty acidDietary FatsMicroRNAs030104 developmental biologyEndocrinologychemistrybiology.proteinIon-channelCALHM1Src kinase030217 neurology & neurosurgery
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The oral lipid sensor GPR120 is not indispensable for the orosensory detectionof dietary lipids in the mouse

2014

International audience; Implication of the long-chain fatty acid (LCFA) receptor GPR120, also termed free fatty acid receptor 4 (FFAR4), in the taste-guided preference for lipids is a matter of debate. To further unravel the role of GPR120 in the "taste of fat", the present study was conducted on GPR120-null mice and their wild-type littermates. Using a combination of morphological (i.e. immunohistochemical staining of circumvallate papillae - CVP), behavioral (i.e. two-bottle preference tests, licking tests and conditioned taste aversion) and functional studies (i.e. calcium imaging in freshly isolated taste bud cells - TBC), we show that absence of GPR120 in oral cavity was not associated…

MaleAgonistmedicine.medical_specialtyTasteG-proteinGPR120Mousemedicine.drug_class[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutritionchemistry.chemical_elementQD415-436CalciumBiologyBiochemistryDiet and dietary lipidsReceptors G-Protein-CoupledFood PreferencesMice03 medical and health sciences0302 clinical medicineEndocrinologyCalcium imagingFeeding behaviorInternal medicineReceptorsmedicineAnimalsReceptorResearch Articles030304 developmental biologyNutritionchemistry.chemical_classification0303 health sciencesFatty acidGPR120Cell BiologyTaste BudsDietary FatsImmunohistochemistryLipids[SDV.AEN] Life Sciences [q-bio]/Food and NutritionEndocrinologychemistryTaste aversionCalciumFat taste[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition030217 neurology & neurosurgeryG proteins
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Concentriols B, C and D, three squalene-type triterpenoids from the ascomycete Daldinia concentrica

2002

Abstract Three squalene-type triterpenoids named concentricols B, C and D ( 1 – 3 ) were isolated from the ethyl acetate extract of fruiting bodies of the xylariaceous ascomycete Daldinia concentrica . Their absolute structures were elucidated by analysis of 2D NMR, MS, IR and UV spectra, and the modified Mosher's method.

SqualeneMagnetic Resonance SpectroscopyMolecular StructureStereochemistrySpectrum AnalysisEthyl acetatePlant ScienceGeneral MedicineHorticultureBiologybiology.organism_classificationBiochemistryTriterpenesSqualenechemistry.chemical_compoundTriterpenoidUv spectraAscomycotachemistryDaldinia concentricaOrganic chemistryMolecular BiologyTwo-dimensional nuclear magnetic resonance spectroscopyPhytochemistry
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