Diversity of Diatrypaceae species in three Spanish grapevine producing regions.

The objective of this study was to identify species of Diatrypaceae associated with trunk diseases in three different Spanish grapevine producing regions: Albacete, Cádiz and Valencia provinces, located, respectively, in geographically distant areas of Central, South and Eastern Spain. Species identification of 71 isolates was performed using a combination of morphological characters and phylogenetic analyses based on the internal transcribed spacer regions of the rDNA (ITS) and the β-tubulin gene. Four species of Diatrypaceae were identified: Cryptovalsa ampelina was the predominant species (63.4%) followed by Eutypa lata (19.7%), Eutypella microtheca (4.2%), and Eutypella citricola (2.8%)…