0000000000447609

AUTHOR

Gaetano Distefano

0000-0002-6080-6466

showing 4 related works from this author

Analysis of S-allele genetic diversity in Sicilian almond germplasm comparing different molecular methods

2015

Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S-RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self-compatible cultivars did not show the presence of Sf allele. Amplicons from consensus and allele-specific PCR primers revealed a high level of variability. Sequencing of all the S-RNase amplicons derived from consensus primers allowed the identification of two new S-RNase alleles (S51 and S52). Surprisingly, despite the AA repl…

GermplasmGeneticsGenetic diversityPoint mutationPrunus dulcis; S-genotyping; self-(in)compatibilityPlant ScienceAmpliconBiologyPrunus dulcisDNA sequencingconsensus and specific primers Prunus dulcis (Miller) self-(in)compatibility S-genotyping S-RNase sequencingSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboreeself-(in)compatibilityGenetic variationS-genotypingGeneticsGene poolAlleleAgronomy and Crop Science
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Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels

2021

AbstractAlmond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfer time-of-flight mass spectrometer and genotyped using the Illumina Infinium®18 K Peach SNP array. The profiling of the aroma was carried out on fresh and roasted kernels enabling the detection of 150 mass peaks. Sixty eight, for the most related with sulfur compounds, furan containing compounds, and aldehydes formed by Strecker degradation, significantly increased during roasting, while …

0106 biological sciences0301 basic medicineGermplasmPlant physiologyPopulationPlant ScienceHorticultureBiology01 natural sciencesBiochemistryArticlePlant breedingAlmond Genetic characterizationTerpene03 medical and health sciencesGeneticsCultivareducationAromaSyntenyRoastingGenetic associationeducation.field_of_studyfood and beveragesbiology.organism_classificationSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticulture030104 developmental biology010606 plant biology & botanyBiotechnology
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Genetic diversity and relationships among Italian and foreign almond germplasm as revealed by microsatellite markers

2013

Abstract Italian germplasm is characterized by a wide diversity rapidly developed determining a massive genetic pool of cultivars in several growing areas. On the whole, regions of southern Italy, and in particular Sicily, were considered as one of the main trade routes along which almond was spread throughout the shores of the Mediterranean Sea. In this work, 9 SSR markers have been used to analyze 113 almond cultivars and accessions coming from ex-situ conservation, including most of almond genotypes spread in Sicily and Apulia and foreign cultivars from Mediterranean, American and Australian areas in order to determine the level of genetic diversity within Italian genotypes and elucidate…

GermplasmCitrusGenotypingGenetic diversityeducation.field_of_studyPhylogenetic treeEcologyPopulationfood and beveragesHorticultureBiologySSRSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeCitrus; Genotyping; SSRCultivated almondGenetic variationBotanyMicrosatelliteGenetic variationGene poolCultivareducationScientia Horticulturae
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Physiological and molecular analysis of the maturation process in fruits of Clementine Mandarin and one of its late-ripening mutants

2009

Peel color is one of the main features affecting citrus quality. Clementine is a widespread citrus species with several mutants showing a delay in pigmentation and harvesting. This work characterizes the fruit development and ripening of two clementine clones, 'Comune', a widespread variety, and one of its natural mutations, 'Tardivo', which differ by a delayed color-break and extended harvest period. Morphological, chemical, and molecular analyses were carried out on fruits of both genotypes during the whole maturation process. Analysis showed that mutation did not affect ripening characteristics such as juice acidity and TSS. However, biochemical and molecular analysis revealed marked dif…

CitrusMutantCitruGene ExpressionBiologychemistry.chemical_compoundPhytoeneethyleneGeneCarotenoidchemistry.chemical_classificationChlorophyllaseAlkyl and Aryl Transferasescarotenogenesifood and beveragesRipeningGeneral ChemistryEthylenesCarotenoidschemistryBiochemistryXanthophyllFruitGeranylgeranyl-Diphosphate GeranylgeranyltransferaseMutationchlorophyllaseHPLCGeneral Agricultural and Biological SciencesClementine
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